TY - JOUR
T1 - Platelet-rich plasma (PRP) and adipose-derived mesenchymal stem cells stimulatory effects on proliferation and migration of fibroblasts and keratinocytes in vitro
AU - Stessuk, Talita
AU - Puzzi, Maria Beatriz
AU - Chaim, Elinton Adami
AU - Alves, Paulo César Martins
AU - de Paula, Erich Vinicius
AU - Forte, Andresa
AU - Izumizawa, Juliana Massae
AU - Oliveira, Carolina Caliári
AU - Frei, Fernando
AU - Ribeiro-Paes, João Tadeu
PY - 2016/9/1
Y1 - 2016/9/1
N2 - The clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo.
AB - The clinical use of tissue engineering associated with cell therapy is considered a new alternative therapy for the repair of chronic lesions with potential application in different medical areas, mostly in orthopedic and dermatological diseases. Platelet-rich plasma (PRP) is a rich source of growth factors and cytokines important for wound healing. Adipose-derived mesenchymal stem cells (ADSCs) have shown potential to accelerate the resolution of ulcers, to stimulate cell proliferation, and to benefit the quality of skin repair. This study aims to determine the effect of PRP and conditioned medium (CM) from ADSC on fibroblast and keratinocyte proliferation in vitro. Migration and proliferation assays were performed to evaluate the growth of fibroblasts and keratinocytes in the presence of PRP, CM, and CM + PRP. Significant proliferative stimulation was observed after 48 h of culture (p < 0.05) on mean absorbance of fibroblasts cultured with 10 and 25 % PRP, 100 % CM, and 25 % PRP + 25 % CM, if compared with control. Keratinocyte proliferation was stimulated after 48 h in cultures with 25, 50, and 100 % CM, and growth was compared with controls. The migration assay detected a significant migratory stimulus in fibroblasts cultured with 10 % PRP + 10 % CM after 48 h. These in vitro results suggest that PRP and ADSC have therapeutic potential for healing and re-epithelialization of chronic wounds in vivo.
KW - Fibroblasts
KW - Keratinocytes
KW - Mesenchymal stem cells
KW - Platelet-rich plasma
UR - http://www.scopus.com/inward/record.url?scp=84978036278&partnerID=8YFLogxK
U2 - 10.1007/s00403-016-1676-1
DO - 10.1007/s00403-016-1676-1
M3 - Article
C2 - 27394438
AN - SCOPUS:84978036278
SN - 0340-3696
VL - 308
SP - 511
EP - 520
JO - Archives of Dermatological Research
JF - Archives of Dermatological Research
IS - 7
ER -