Plasmon-enhanced single-molecule enzymology

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)
115 Downloads (Pure)


We present a numerical study on plasmon-enhanced single-molecule enzymology. We combine Brownian dynamics and electromagnetic simulations to calculate the enhancement of fluorescence signals of fluorogenic substrate converted by an enzyme conjugated to a plasmonic particle. We simulate the Brownian motion of a fluorescent product away from the active site of the enzyme, and calculate the photon detection rate taking into account modifications of the excitation and emission processes by coupling to the plasmon. We show that plasmon enhancement can boost the signal-to-noise ratio (SNR) of single turnovers by up to 100 fold compared to confocal microscopy. This enhancement factor is a trade-off between the reduced residence time in the near-field of the particle, and the enhanced emission intensity due to coupling to the plasmon. The enhancement depends on the size, shape and material of the particle and the photophysical properties of the fluorescent product. Our study provides guidelines on how to enhance the SNR of single-molecule enzyme studies and may aid in further understanding and quantifying static and dynamic heterogeneity.
Original languageEnglish
Pages (from-to)3073–3081
Number of pages9
JournalACS Photonics
Issue number8
Publication statusPublished - 15 Aug 2018


  • Brownian motion
  • Plasmonic nanoantennas
  • numerical modeling, gold nanorods
  • plasmon-enhanced fluorescence
  • single-molecule enzymology
  • gold nanorods
  • numerical modeling


Dive into the research topics of 'Plasmon-enhanced single-molecule enzymology'. Together they form a unique fingerprint.

Cite this