Pixe microanalysis of trace elements of normoxic, isochemic and reperfused rat hearts

B.A.W. Verhoef; J.J.M. de Goeij; P.H.A. Mutsaers; M.J.A. Voigt, de; P.H.H. Bomans; P.M. Frederik; G.J. Vusse, van der

Pixe microanalysis of trace elements of normoxic, isochemic and reperfused rat hearts

B.A.W. Verhoef, J.J.M. de Goeij, P.H.A. Mutsaers, M.J.A. Voigt, de, P.H.H. Bomans, P.M. Frederik, G.J. Vusse, van der

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Abstract

With a 3 x 3 J.1.rn2 proton microbearn spatial distributions of Na, Mg; P, S, K, Ca and Fe were measured via PIXE in 50 x 50 J.1.rn2 areas of rat heart, sliced into 1~15 J.1.rn thick ryosections. The isolated rat hearts were subjected to normal perfusion, ischemia and reperfusion. Substantial changes in the elemental distribution were found in tissue after 40 min. of reperfusion, particularly indicated by locally elevated Ca and decreased K levels. Electron microscopic examination was used for assessment of artefacts due to sample preparation and handling. Results of stained cryosections analyzed via STIM demonstrated that this latter technique can be employed prior to PIXE analysis to localize individual cells in freeze-dried cryosections.

Original language	English
Pages (from-to)	59-67
Number of pages	9
Journal	Cellular and Molecular Biology
Volume	42
Issue number	1
Publication status	Published - 1996

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title = "Pixe microanalysis of trace elements of normoxic, isochemic and reperfused rat hearts",

abstract = "With a 3 x 3 J.1.rn2 proton microbearn spatial distributions of Na, Mg; P, S, K, Ca and Fe were measured via PIXE in 50 x 50 J.1.rn2 areas of rat heart, sliced into 1~15 J.1.rn thick ryosections. The isolated rat hearts were subjected to normal perfusion, ischemia and reperfusion. Substantial changes in the elemental distribution were found in tissue after 40 min. of reperfusion, particularly indicated by locally elevated Ca and decreased K levels. Electron microscopic examination was used for assessment of artefacts due to sample preparation and handling. Results of stained cryosections analyzed via STIM demonstrated that this latter technique can be employed prior to PIXE analysis to localize individual cells in freeze-dried cryosections.",

author = "B.A.W. Verhoef and {de Goeij}, J.J.M. and P.H.A. Mutsaers and {Voigt, de}, M.J.A. and P.H.H. Bomans and P.M. Frederik and {Vusse, van der}, G.J.",

year = "1996",

language = "English",

volume = "42",

pages = "59--67",

journal = "Cellular and Molecular Biology",

issn = "0145-5680",

publisher = "Cellular and Molecular Biology Association",

number = "1",

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TY - JOUR

T1 - Pixe microanalysis of trace elements of normoxic, isochemic and reperfused rat hearts

AU - Verhoef, B.A.W.

AU - de Goeij, J.J.M.

AU - Mutsaers, P.H.A.

AU - Voigt, de, M.J.A.

AU - Bomans, P.H.H.

AU - Frederik, P.M.

AU - Vusse, van der, G.J.

PY - 1996

Y1 - 1996

N2 - With a 3 x 3 J.1.rn2 proton microbearn spatial distributions of Na, Mg; P, S, K, Ca and Fe were measured via PIXE in 50 x 50 J.1.rn2 areas of rat heart, sliced into 1~15 J.1.rn thick ryosections. The isolated rat hearts were subjected to normal perfusion, ischemia and reperfusion. Substantial changes in the elemental distribution were found in tissue after 40 min. of reperfusion, particularly indicated by locally elevated Ca and decreased K levels. Electron microscopic examination was used for assessment of artefacts due to sample preparation and handling. Results of stained cryosections analyzed via STIM demonstrated that this latter technique can be employed prior to PIXE analysis to localize individual cells in freeze-dried cryosections.

AB - With a 3 x 3 J.1.rn2 proton microbearn spatial distributions of Na, Mg; P, S, K, Ca and Fe were measured via PIXE in 50 x 50 J.1.rn2 areas of rat heart, sliced into 1~15 J.1.rn thick ryosections. The isolated rat hearts were subjected to normal perfusion, ischemia and reperfusion. Substantial changes in the elemental distribution were found in tissue after 40 min. of reperfusion, particularly indicated by locally elevated Ca and decreased K levels. Electron microscopic examination was used for assessment of artefacts due to sample preparation and handling. Results of stained cryosections analyzed via STIM demonstrated that this latter technique can be employed prior to PIXE analysis to localize individual cells in freeze-dried cryosections.

M3 - Article

SN - 0145-5680

VL - 42

SP - 59

EP - 67

JO - Cellular and Molecular Biology

JF - Cellular and Molecular Biology

IS - 1

ER -

Pixe microanalysis of trace elements of normoxic, isochemic and reperfused rat hearts

Abstract

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