Phospholipid asymmetry of the outer membrane of rat liver mitochondria : evidence for the presence of cardiolipin on the outside of the outer membrane

R. Hovius; J. Thijssen; P. Linden, van der; K. Nicolaij; B. Kruijff, de

doi:10.1016/0014-5793(93)80922-H

Phospholipid asymmetry of the outer membrane of rat liver mitochondria : evidence for the presence of cardiolipin on the outside of the outer membrane

R. Hovius, J. Thijssen, P. Linden, van der, K. Nicolaij, B. Kruijff, de

Mechanical Engineering

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The phospholipid topology of the outer membrane of intact rat liver mitochondria and derived outer membrane vesicles was investigated by determining the accessible pool of the various phospholipid classes towards phospholipase A2, a phosphatidylcholine-specific transfer protein and by chemical labeling using trinitrobenzene sulfonic acid. The outer membrane vesicles are sealed and have a right-side-out topology with a proposed localization of 55%, 77%, 100%, and at least 30% of the phosphatidylcholine, phosphatidylethanolamine, cardiolipin, and phosphatidylinositol plus phosphatidylserine in the outer leaflet, respectively. The outer membrane in intact mitochondria appears to have a similar phospholipid distribution.

Original language	English
Pages (from-to)	71-76
Number of pages	6
Journal	FEBS Letters
Volume	330
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(93)80922-H
Publication status	Published - 1993

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title = "Phospholipid asymmetry of the outer membrane of rat liver mitochondria : evidence for the presence of cardiolipin on the outside of the outer membrane",

abstract = "The phospholipid topology of the outer membrane of intact rat liver mitochondria and derived outer membrane vesicles was investigated by determining the accessible pool of the various phospholipid classes towards phospholipase A2, a phosphatidylcholine-specific transfer protein and by chemical labeling using trinitrobenzene sulfonic acid. The outer membrane vesicles are sealed and have a right-side-out topology with a proposed localization of 55%, 77%, 100%, and at least 30% of the phosphatidylcholine, phosphatidylethanolamine, cardiolipin, and phosphatidylinositol plus phosphatidylserine in the outer leaflet, respectively. The outer membrane in intact mitochondria appears to have a similar phospholipid distribution.",

author = "R. Hovius and J. Thijssen and {Linden, van der}, P. and K. Nicolaij and {Kruijff, de}, B.",

year = "1993",

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AU - Hovius, R.

AU - Thijssen, J.

AU - Linden, van der, P.

AU - Nicolaij, K.

AU - Kruijff, de, B.

PY - 1993

Y1 - 1993

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AB - The phospholipid topology of the outer membrane of intact rat liver mitochondria and derived outer membrane vesicles was investigated by determining the accessible pool of the various phospholipid classes towards phospholipase A2, a phosphatidylcholine-specific transfer protein and by chemical labeling using trinitrobenzene sulfonic acid. The outer membrane vesicles are sealed and have a right-side-out topology with a proposed localization of 55%, 77%, 100%, and at least 30% of the phosphatidylcholine, phosphatidylethanolamine, cardiolipin, and phosphatidylinositol plus phosphatidylserine in the outer leaflet, respectively. The outer membrane in intact mitochondria appears to have a similar phospholipid distribution.

U2 - 10.1016/0014-5793(93)80922-H

DO - 10.1016/0014-5793(93)80922-H

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JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Phospholipid asymmetry of the outer membrane of rat liver mitochondria : evidence for the presence of cardiolipin on the outside of the outer membrane

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