Phospholipid asymmetry of the outer membrane of rat liver mitochondria : evidence for the presence of cardiolipin on the outside of the outer membrane

R. Hovius, J. Thijssen, P. Linden, van der, K. Nicolaij, B. Kruijff, de

Research output: Contribution to journalArticleAcademicpeer-review

80 Citations (Scopus)

Abstract

The phospholipid topology of the outer membrane of intact rat liver mitochondria and derived outer membrane vesicles was investigated by determining the accessible pool of the various phospholipid classes towards phospholipase A2, a phosphatidylcholine-specific transfer protein and by chemical labeling using trinitrobenzene sulfonic acid. The outer membrane vesicles are sealed and have a right-side-out topology with a proposed localization of 55%, 77%, 100%, and at least 30% of the phosphatidylcholine, phosphatidylethanolamine, cardiolipin, and phosphatidylinositol plus phosphatidylserine in the outer leaflet, respectively. The outer membrane in intact mitochondria appears to have a similar phospholipid distribution.
Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalFEBS Letters
Volume330
Issue number1
DOIs
Publication statusPublished - 1993

Fingerprint

Dive into the research topics of 'Phospholipid asymmetry of the outer membrane of rat liver mitochondria : evidence for the presence of cardiolipin on the outside of the outer membrane'. Together they form a unique fingerprint.

Cite this