Peroxiredoxin proteins as building blocks for nanotechnology

Frankie Conroy, Amy Yewdall

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Peroxiredoxins are ubiquitous antioxidant proteins that exhibit a striking variety of quaternary structures, making them appealing building blocks with which nanoscale architectures are created for applications in nanotechnology. The solution environment of the protein, as well as protein sequence, influences the presentation of a particular structure, thereby enabling mesoscopic manipulations that affect arrangments at the nanoscale. This chapter will equip us with the knowledge necessary to not only produce and manipulate peroxiredoxin proteins into desired structures but also to characterize the different structures using dynamic light scattering, analytical centrifugation, and negative stain transmission electron microscopy, thereby setting the stage for us to use these proteins for applications in nanotechnology.

Original languageEnglish
Title of host publicationProtein Nanotechnology
EditorsJ. Gerard, L. Domigan
Place of PublicationHumana
PublisherSpringer
Pages39-54
Number of pages16
ISBN (Electronic)978-1-4939-9869-2
ISBN (Print)978-1-4939-9868-5
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2073
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • self-assembly
  • protein
  • Peroxidase
  • Structure function
  • nanotechnology
  • Peroxiredoxin
  • Protein engineering
  • Protein
  • Self-assembly
  • Transmission electron microscopy
  • Dynamic light scattering
  • Prx3
  • Analytical ultracentrifugation
  • Nanotechnology
  • Negative stain

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