TY - JOUR
T1 - Performance evaluation of different design alternatives for microfabricated nonporous fused silica pillar columns for capillary electrochromatography
AU - Sukas, Sertan
AU - De Malsche, Wim
AU - Desmet, Gert
AU - Gardeniers, Han J.G.E.
PY - 2012/11/20
Y1 - 2012/11/20
N2 - An experimental study comparing the performance of different designs for microfabricated column structures for microchip capillary electrochromatography is presented. The work is a follow-up to our previously published modeling and simulation study on the same topic. Experiments were performed using fused silica microchips with and without octadecyltrimethoxysilane coating for nonretained and retained modes of operation, respectively. Showing the same trends as the modeling results, the foil shape produces a significant decrease in plate height with an increase of around 15% in mobile phase velocity in nonretained measurements of Coumarin 480 (C480). Measured plate heights at 1 kV/cm applied electric field were 0.77, 1.33, and 1.42 μm for foil, diamond, and hexagon, respectively. Chromatographic runs of C480 yielded minimal plate height values of 1.85 and 3.28 μm for foil and diamond, respectively. The optimization of the shape and placement of the structures appeared to have a considerable impact on the achievable performance.
AB - An experimental study comparing the performance of different designs for microfabricated column structures for microchip capillary electrochromatography is presented. The work is a follow-up to our previously published modeling and simulation study on the same topic. Experiments were performed using fused silica microchips with and without octadecyltrimethoxysilane coating for nonretained and retained modes of operation, respectively. Showing the same trends as the modeling results, the foil shape produces a significant decrease in plate height with an increase of around 15% in mobile phase velocity in nonretained measurements of Coumarin 480 (C480). Measured plate heights at 1 kV/cm applied electric field were 0.77, 1.33, and 1.42 μm for foil, diamond, and hexagon, respectively. Chromatographic runs of C480 yielded minimal plate height values of 1.85 and 3.28 μm for foil and diamond, respectively. The optimization of the shape and placement of the structures appeared to have a considerable impact on the achievable performance.
UR - http://www.scopus.com/inward/record.url?scp=84869441335&partnerID=8YFLogxK
U2 - 10.1021/ac302450z
DO - 10.1021/ac302450z
M3 - Article
AN - SCOPUS:84869441335
SN - 0003-2700
VL - 84
SP - 9996
EP - 10004
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 22
ER -