Pathway of propionate formation in Desulfobulbus propionicus

A.J.M. Stams, D.R. Kremer, K. Nicolaij, G. Weenk, T.A. Hansen

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Abstract

Whole cells of Desulfobulbus propionicus fermented [1-13C]ethanol to [2-13C] and [3-13C]propionate and [1-13C]-acetate, which indicates the involvement of a randomizing pathway in the formation of propionate. Cell-free extracts prepared from cells grown on lactate (without sulfate) contained high activities of methylmalonyl-CoA: pyruvate transacetylase, acetase kinase and reasonably high activities of NAD(P)-independent L(+)-lactate dehydrogenase NAD(P)-independent pyruvate dehydrogenase, phosphotransacetylase, acetate kinase and reasonably high activity of NAD(P)-independent L(+)-lactate dehydrogenase, fumarate reductase and succinate dehydrogenase. Cell-free extracts catalyzed the conversion of succinate to propionate in the presence of pyruvate, CoA and ATP and the oxaloacetate-dependent conversion of propionate to succinate. After growth on lactate or propionate in the presence of sulfate similar enzyme levels were found except for fumarate reductase which was considerably lower. Fermentative growth on lactate led to higher cytochrome b contents than growth with sulfate as electron acceptor. The labeling studies and the enzyme measurements demonstrate that in Desulfobulbus propionate is formed via a succinate pathway involving a transcarboxylase like in Propionibacterium. The same pathway may be used for the degradation of propionate to acetate in the presence of sulfate.
Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalArchives of Microbiology
Volume139
Issue number2-3
DOIs
Publication statusPublished - 1984

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Propionates
Sulfates
Succinate Dehydrogenase
Succinic Acid
Pyruvic Acid
NAD
Lactic Acid
Methylmalonyl-CoA carboxytransferase
Cell Extracts
L-Lactate Dehydrogenase
Acetates
Phosphate Acetyltransferase
Acetate Kinase
Growth
Propionibacterium
Oxaloacetic Acid
Cytochromes b
Enzymes
Coenzyme A
Labeling

Cite this

Stams, A. J. M., Kremer, D. R., Nicolaij, K., Weenk, G., & Hansen, T. A. (1984). Pathway of propionate formation in Desulfobulbus propionicus. Archives of Microbiology, 139(2-3), 167-173. https://doi.org/10.1007/BF00401994
Stams, A.J.M. ; Kremer, D.R. ; Nicolaij, K. ; Weenk, G. ; Hansen, T.A. / Pathway of propionate formation in Desulfobulbus propionicus. In: Archives of Microbiology. 1984 ; Vol. 139, No. 2-3. pp. 167-173.
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Stams, AJM, Kremer, DR, Nicolaij, K, Weenk, G & Hansen, TA 1984, 'Pathway of propionate formation in Desulfobulbus propionicus', Archives of Microbiology, vol. 139, no. 2-3, pp. 167-173. https://doi.org/10.1007/BF00401994

Pathway of propionate formation in Desulfobulbus propionicus. / Stams, A.J.M.; Kremer, D.R.; Nicolaij, K.; Weenk, G.; Hansen, T.A.

In: Archives of Microbiology, Vol. 139, No. 2-3, 1984, p. 167-173.

Research output: Contribution to journalArticleAcademicpeer-review

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AB - Whole cells of Desulfobulbus propionicus fermented [1-13C]ethanol to [2-13C] and [3-13C]propionate and [1-13C]-acetate, which indicates the involvement of a randomizing pathway in the formation of propionate. Cell-free extracts prepared from cells grown on lactate (without sulfate) contained high activities of methylmalonyl-CoA: pyruvate transacetylase, acetase kinase and reasonably high activities of NAD(P)-independent L(+)-lactate dehydrogenase NAD(P)-independent pyruvate dehydrogenase, phosphotransacetylase, acetate kinase and reasonably high activity of NAD(P)-independent L(+)-lactate dehydrogenase, fumarate reductase and succinate dehydrogenase. Cell-free extracts catalyzed the conversion of succinate to propionate in the presence of pyruvate, CoA and ATP and the oxaloacetate-dependent conversion of propionate to succinate. After growth on lactate or propionate in the presence of sulfate similar enzyme levels were found except for fumarate reductase which was considerably lower. Fermentative growth on lactate led to higher cytochrome b contents than growth with sulfate as electron acceptor. The labeling studies and the enzyme measurements demonstrate that in Desulfobulbus propionate is formed via a succinate pathway involving a transcarboxylase like in Propionibacterium. The same pathway may be used for the degradation of propionate to acetate in the presence of sulfate.

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Stams AJM, Kremer DR, Nicolaij K, Weenk G, Hansen TA. Pathway of propionate formation in Desulfobulbus propionicus. Archives of Microbiology. 1984;139(2-3):167-173. https://doi.org/10.1007/BF00401994