Painting supramolecular polymers in organic solvents by super-resolution microscopy

Beatrice Adelizzi, Antonio Aloi, Nathan J. Van Zee, Anja R.A. Palmans, E.W. Meijer, Ilja K. Voets

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)
80 Downloads (Pure)

Abstract

Despite the rapid development of complex functional supramolecular systems, visualization of these architectures under native conditions at high resolution has remained a challenging endeavor. Super-resolution microscopy was recently proposed as an effective tool to unveil one-dimensional nanoscale structures in aqueous media upon chemical functionalization with suitable fluorescent probes. Building upon our previous work, which enabled photoactivation localization microscopy in organic solvents, herein, we present the imaging of one-dimensional supramolecular polymers in their native environment by interface point accumulation for imaging in nanoscale topography (iPAINT). The noncovalent staining, typical of iPAINT, allows the investigation of supramolecular polymers' structure in situ without any chemical modification. The quasi-permanent adsorption of the dye to the polymer is exploited to identify block-like arrangements within supramolecular fibers, which were obtained upon mixing homopolymers that were prestained with different colors. The staining of the blocks, maintained by the lack of exchange of the dyes, permits the imaging of complex structures for multiple days. This study showcases the potential of PAINT-like strategies such as iPAINT to visualize multicomponent dynamic systems in their native environment with an easy, synthesis-free approach and high spatial resolution.

Original languageEnglish
Pages (from-to)4431-4439
Number of pages9
JournalACS Nano
Volume12
Issue number5
DOIs
Publication statusPublished - 22 May 2018

Fingerprint

Painting
Organic solvents
Microscopic examination
topography
Polymers
staining
microscopy
Imaging techniques
Topography
polymers
dyes
high resolution
Coloring Agents
Dyes
spatial resolution
Chemical modification
color
Homopolymerization
adsorption
Fluorescent Dyes

Keywords

  • iPAINT
  • multicomponent unidimensional aggregates
  • self-assembly
  • single-molecule localization microscopy
  • super-resolution microscopy
  • supramolecular block copolymers

Cite this

@article{3c9fb5e68d324954bc69045136203a26,
title = "Painting supramolecular polymers in organic solvents by super-resolution microscopy",
abstract = "Despite the rapid development of complex functional supramolecular systems, visualization of these architectures under native conditions at high resolution has remained a challenging endeavor. Super-resolution microscopy was recently proposed as an effective tool to unveil one-dimensional nanoscale structures in aqueous media upon chemical functionalization with suitable fluorescent probes. Building upon our previous work, which enabled photoactivation localization microscopy in organic solvents, herein, we present the imaging of one-dimensional supramolecular polymers in their native environment by interface point accumulation for imaging in nanoscale topography (iPAINT). The noncovalent staining, typical of iPAINT, allows the investigation of supramolecular polymers' structure in situ without any chemical modification. The quasi-permanent adsorption of the dye to the polymer is exploited to identify block-like arrangements within supramolecular fibers, which were obtained upon mixing homopolymers that were prestained with different colors. The staining of the blocks, maintained by the lack of exchange of the dyes, permits the imaging of complex structures for multiple days. This study showcases the potential of PAINT-like strategies such as iPAINT to visualize multicomponent dynamic systems in their native environment with an easy, synthesis-free approach and high spatial resolution.",
keywords = "iPAINT, multicomponent unidimensional aggregates, self-assembly, single-molecule localization microscopy, super-resolution microscopy, supramolecular block copolymers",
author = "Beatrice Adelizzi and Antonio Aloi and {Van Zee}, {Nathan J.} and Palmans, {Anja R.A.} and E.W. Meijer and Voets, {Ilja K.}",
year = "2018",
month = "5",
day = "22",
doi = "10.1021/acsnano.8b00396",
language = "English",
volume = "12",
pages = "4431--4439",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "5",

}

Painting supramolecular polymers in organic solvents by super-resolution microscopy. / Adelizzi, Beatrice; Aloi, Antonio; Van Zee, Nathan J.; Palmans, Anja R.A.; Meijer, E.W.; Voets, Ilja K.

In: ACS Nano, Vol. 12, No. 5, 22.05.2018, p. 4431-4439.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Painting supramolecular polymers in organic solvents by super-resolution microscopy

AU - Adelizzi, Beatrice

AU - Aloi, Antonio

AU - Van Zee, Nathan J.

AU - Palmans, Anja R.A.

AU - Meijer, E.W.

AU - Voets, Ilja K.

PY - 2018/5/22

Y1 - 2018/5/22

N2 - Despite the rapid development of complex functional supramolecular systems, visualization of these architectures under native conditions at high resolution has remained a challenging endeavor. Super-resolution microscopy was recently proposed as an effective tool to unveil one-dimensional nanoscale structures in aqueous media upon chemical functionalization with suitable fluorescent probes. Building upon our previous work, which enabled photoactivation localization microscopy in organic solvents, herein, we present the imaging of one-dimensional supramolecular polymers in their native environment by interface point accumulation for imaging in nanoscale topography (iPAINT). The noncovalent staining, typical of iPAINT, allows the investigation of supramolecular polymers' structure in situ without any chemical modification. The quasi-permanent adsorption of the dye to the polymer is exploited to identify block-like arrangements within supramolecular fibers, which were obtained upon mixing homopolymers that were prestained with different colors. The staining of the blocks, maintained by the lack of exchange of the dyes, permits the imaging of complex structures for multiple days. This study showcases the potential of PAINT-like strategies such as iPAINT to visualize multicomponent dynamic systems in their native environment with an easy, synthesis-free approach and high spatial resolution.

AB - Despite the rapid development of complex functional supramolecular systems, visualization of these architectures under native conditions at high resolution has remained a challenging endeavor. Super-resolution microscopy was recently proposed as an effective tool to unveil one-dimensional nanoscale structures in aqueous media upon chemical functionalization with suitable fluorescent probes. Building upon our previous work, which enabled photoactivation localization microscopy in organic solvents, herein, we present the imaging of one-dimensional supramolecular polymers in their native environment by interface point accumulation for imaging in nanoscale topography (iPAINT). The noncovalent staining, typical of iPAINT, allows the investigation of supramolecular polymers' structure in situ without any chemical modification. The quasi-permanent adsorption of the dye to the polymer is exploited to identify block-like arrangements within supramolecular fibers, which were obtained upon mixing homopolymers that were prestained with different colors. The staining of the blocks, maintained by the lack of exchange of the dyes, permits the imaging of complex structures for multiple days. This study showcases the potential of PAINT-like strategies such as iPAINT to visualize multicomponent dynamic systems in their native environment with an easy, synthesis-free approach and high spatial resolution.

KW - iPAINT

KW - multicomponent unidimensional aggregates

KW - self-assembly

KW - single-molecule localization microscopy

KW - super-resolution microscopy

KW - supramolecular block copolymers

UR - http://www.scopus.com/inward/record.url?scp=85046540645&partnerID=8YFLogxK

U2 - 10.1021/acsnano.8b00396

DO - 10.1021/acsnano.8b00396

M3 - Article

C2 - 29697958

AN - SCOPUS:85046540645

VL - 12

SP - 4431

EP - 4439

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 5

ER -