Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials

Mireille M.J.P.E. Sthijns; Marlon J. Jetten; Sami G. Mohammed; Sandra M.H. Claessen; Rick H.W. de Vries; Adam Stell; Denise F.A. de Bont; Marten A. Engelse; Didem Mumcuoglu; Clemens A. van Blitterswijk; Patricia Y.W. Dankers; Eelco J.P. de Koning; Aart A. van Apeldoorn; Vanessa L.S. LaPointe

doi:10.1016/j.biomaterials.2020.120449

Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials

Mireille M.J.P.E. Sthijns
, Marlon J. Jetten
, Sami G. Mohammed
, Sandra M.H. Claessen
, Rick H.W. de Vries
, Adam Stell
, Denise F.A. de Bont
, Marten A. Engelse
, Didem Mumcuoglu
, Clemens A. van Blitterswijk
, Patricia Y.W. Dankers
, Eelco J.P. de Koning
, Aart A. van Apeldoorn
, Vanessa L.S. LaPointe (Corresponding author)

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function. We found that 300 poly(ethylene oxide terephthalate) 55/poly(butylene terephthalate) 45 (PEOT/PBT300) was more resistant to breakage and more elastic than other biomaterials, which is important for its immunoprotective function. In addition, it did not induce oxidative stress or reduce viability in the MIN6 beta cell line, and even promoted protective endogenous antioxidant expression over 7 days. Importantly, PEOT/PBT300 is one of the biomaterials we studied that did not interfere with insulin secretion in human islets.

Original language	English
Article number	120449
Number of pages	13
Journal	Biomaterials
Volume	267
DOIs	https://doi.org/10.1016/j.biomaterials.2020.120449
Publication status	Published - Jan 2021

Funding

This research has received funding from the Dutch Province of Limburg, from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 694801 ), and from the Juvenile Diabetes Research Foundation (grant agreement No 3-SRA-2016-256-S-B and No 3-SRA-2017-428-S-B ). In addition, it is supported by the partners of Regenerative Medicine Crossing Borders (RegMed XB), a public–private partnership that uses regenerative medicine strategies to cure common chronic diseases. This collaboration project is financed by the Dutch Ministry of Economic Affairs by means of the PPP Allowance made available by the Top Sector Life Sciences & Health to stimulate public–private partnerships. The funding sources had no involvement in the study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the article for publication. We would like to thank Tonny Bosman (SupraPolix, the Netherlands) for providing us the UPy-PC material and Hang Nguyen (Maastricht University, the Netherlands) for her help revising the manuscript.

Funders	Funder number
European Union's Horizon 2020 - Research and Innovation Framework Programme
Ministerie van Economische Zaken en Klimaat
European Union's Horizon 2020 - Research and Innovation Framework Programme	694801

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biomaterials
Clinical islet transplantation
Islet encapsulation device
Oxidative stress
Type 1 diabetes

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10.1016/j.biomaterials.2020.120449

Cite this

Sthijns, M. M. J. P. E., Jetten, M. J., Mohammed, S. G., Claessen, S. M. H., de Vries, R. H. W., Stell, A., de Bont, D. F. A., Engelse, M. A., Mumcuoglu, D., van Blitterswijk, C. A., Dankers, P. Y. W., de Koning, E. J. P., van Apeldoorn, A. A., & LaPointe, V. L. S. (2021). Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials. Biomaterials, 267, Article 120449. https://doi.org/10.1016/j.biomaterials.2020.120449

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title = "Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials",

abstract = "The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function. We found that 300 poly(ethylene oxide terephthalate) 55/poly(butylene terephthalate) 45 (PEOT/PBT300) was more resistant to breakage and more elastic than other biomaterials, which is important for its immunoprotective function. In addition, it did not induce oxidative stress or reduce viability in the MIN6 beta cell line, and even promoted protective endogenous antioxidant expression over 7 days. Importantly, PEOT/PBT300 is one of the biomaterials we studied that did not interfere with insulin secretion in human islets.",

keywords = "Biomaterials, Clinical islet transplantation, Islet encapsulation device, Oxidative stress, Type 1 diabetes",

author = "Sthijns, \{Mireille M.J.P.E.\} and Jetten, \{Marlon J.\} and Mohammed, \{Sami G.\} and Claessen, \{Sandra M.H.\} and \{de Vries\}, \{Rick H.W.\} and Adam Stell and \{de Bont\}, \{Denise F.A.\} and Engelse, \{Marten A.\} and Didem Mumcuoglu and \{van Blitterswijk\}, \{Clemens A.\} and Dankers, \{Patricia Y.W.\} and \{de Koning\}, \{Eelco J.P.\} and \{van Apeldoorn\}, \{Aart A.\} and LaPointe, \{Vanessa L.S.\}",

year = "2021",

month = jan,

doi = "10.1016/j.biomaterials.2020.120449",

language = "English",

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Sthijns, MMJPE, Jetten, MJ, Mohammed, SG, Claessen, SMH, de Vries, RHW, Stell, A, de Bont, DFA, Engelse, MA, Mumcuoglu, D, van Blitterswijk, CA, Dankers, PYW, de Koning, EJP, van Apeldoorn, AA & LaPointe, VLS 2021, 'Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials', Biomaterials, vol. 267, 120449. https://doi.org/10.1016/j.biomaterials.2020.120449

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AU - Sthijns, Mireille M.J.P.E.

AU - Jetten, Marlon J.

AU - Mohammed, Sami G.

AU - Claessen, Sandra M.H.

AU - de Vries, Rick H.W.

AU - Stell, Adam

AU - de Bont, Denise F.A.

AU - Engelse, Marten A.

AU - Mumcuoglu, Didem

AU - van Blitterswijk, Clemens A.

AU - Dankers, Patricia Y.W.

AU - de Koning, Eelco J.P.

AU - van Apeldoorn, Aart A.

AU - LaPointe, Vanessa L.S.

PY - 2021/1

Y1 - 2021/1

N2 - The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function. We found that 300 poly(ethylene oxide terephthalate) 55/poly(butylene terephthalate) 45 (PEOT/PBT300) was more resistant to breakage and more elastic than other biomaterials, which is important for its immunoprotective function. In addition, it did not induce oxidative stress or reduce viability in the MIN6 beta cell line, and even promoted protective endogenous antioxidant expression over 7 days. Importantly, PEOT/PBT300 is one of the biomaterials we studied that did not interfere with insulin secretion in human islets.

AB - The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function. We found that 300 poly(ethylene oxide terephthalate) 55/poly(butylene terephthalate) 45 (PEOT/PBT300) was more resistant to breakage and more elastic than other biomaterials, which is important for its immunoprotective function. In addition, it did not induce oxidative stress or reduce viability in the MIN6 beta cell line, and even promoted protective endogenous antioxidant expression over 7 days. Importantly, PEOT/PBT300 is one of the biomaterials we studied that did not interfere with insulin secretion in human islets.

KW - Biomaterials

KW - Clinical islet transplantation

KW - Islet encapsulation device

KW - Oxidative stress

KW - Type 1 diabetes

UR - https://www.scopus.com/pages/publications/85094218232

U2 - 10.1016/j.biomaterials.2020.120449

DO - 10.1016/j.biomaterials.2020.120449

M3 - Article

C2 - 33129188

AN - SCOPUS:85094218232

SN - 0142-9612

VL - 267

JO - Biomaterials

JF - Biomaterials

M1 - 120449

ER -

Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials

Abstract

Funding

UN SDGs

Keywords

Access to Document

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