Optimization of the separation of the Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides

A.J.J.M. Coenen; L.H.G. Henckens; Y. Mengerink; S.J. Wal, van der; P.J.L.M. Quaedflieg; L.H. Koole; E.M. Meijer

doi:10.1016/0021-9673(92)80202-6

Optimization of the separation of the Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides

A.J.J.M. Coenen, L.H.G. Henckens, Y. Mengerink, S.J. Wal, van der, P.J.L.M. Quaedflieg, L.H. Koole, E.M. Meijer

Macromolecular and Organic Chemistry

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Abstract

The separation by reversed-phase high-performance liquid chromatography of Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides was studied with respect to pH, organic modifier type and concentration and reversed-phase packing material. Drylab G was used to deduce optimum conditions. On the basis of the observed discrepancies between the computer predictions and experimental results, the gradient operation procedure with volatile buffers was improved. By repetitive chromatography on a 250 × 22 mm I.D. reversed-phase column, fourteen diastereomeric pairs were obtained in at least 97% purity and 60% yield, in amounts of 10–100 mg.

Original language	English
Pages (from-to)	59-66
Journal	Journal of Chromatography
Volume	596
Issue number	1
DOIs	https://doi.org/10.1016/0021-9673(92)80202-6
Publication status	Published - 1992

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title = "Optimization of the separation of the Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides",

abstract = "The separation by reversed-phase high-performance liquid chromatography of Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides was studied with respect to pH, organic modifier type and concentration and reversed-phase packing material. Drylab G was used to deduce optimum conditions. On the basis of the observed discrepancies between the computer predictions and experimental results, the gradient operation procedure with volatile buffers was improved. By repetitive chromatography on a 250 × 22 mm I.D. reversed-phase column, fourteen diastereomeric pairs were obtained in at least 97% purity and 60% yield, in amounts of 10–100 mg.",

author = "A.J.J.M. Coenen and L.H.G. Henckens and Y. Mengerink and {Wal, van der}, S.J. and P.J.L.M. Quaedflieg and L.H. Koole and E.M. Meijer",

year = "1992",

doi = "10.1016/0021-9673(92)80202-6",

language = "English",

volume = "596",

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T1 - Optimization of the separation of the Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides

AU - Coenen, A.J.J.M.

AU - Henckens, L.H.G.

AU - Mengerink, Y.

AU - Wal, van der, S.J.

AU - Quaedflieg, P.J.L.M.

AU - Koole, L.H.

AU - Meijer, E.M.

PY - 1992

Y1 - 1992

N2 - The separation by reversed-phase high-performance liquid chromatography of Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides was studied with respect to pH, organic modifier type and concentration and reversed-phase packing material. Drylab G was used to deduce optimum conditions. On the basis of the observed discrepancies between the computer predictions and experimental results, the gradient operation procedure with volatile buffers was improved. By repetitive chromatography on a 250 × 22 mm I.D. reversed-phase column, fourteen diastereomeric pairs were obtained in at least 97% purity and 60% yield, in amounts of 10–100 mg.

AB - The separation by reversed-phase high-performance liquid chromatography of Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides was studied with respect to pH, organic modifier type and concentration and reversed-phase packing material. Drylab G was used to deduce optimum conditions. On the basis of the observed discrepancies between the computer predictions and experimental results, the gradient operation procedure with volatile buffers was improved. By repetitive chromatography on a 250 × 22 mm I.D. reversed-phase column, fourteen diastereomeric pairs were obtained in at least 97% purity and 60% yield, in amounts of 10–100 mg.

U2 - 10.1016/0021-9673(92)80202-6

DO - 10.1016/0021-9673(92)80202-6

M3 - Article

SN - 0021-9673

VL - 596

SP - 59

EP - 66

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 1

ER -

Optimization of the separation of the Rp and Sp diastereomers of phosphate-methylated DNA and RNA dinucleotides

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