A method has been developed and evaluated for optimization of the selectivity of series-coupled capillary columns in a twin-ove gas chromatographic system in the shortest possible analysis time. The temperatures of either one or both columns were varied independently (partial optimization) or simultaneously (overall optimization), in order to separate the maximum number of sample components. The method isb ased upon computer-aided calculation of Kováts indices of the coupled columns by means of seconsd and third order (polynomial) functions of the temperatures of the individual columns. The applicability and limitations of the methox are discussed and illustrated with separations of a synthetic hydrocarbon mixture of 33 components.
Repka, D., Krupcik, J., Benicka, E., Leclercq, P. A., & Rijks, J. A. (1989). Optimization of selectivity by tuning column temperatures for series-coupled capillary columns in dual-oven gas chromatographic systems. Journal of Chromatography, A, 463(2), 243-251. https://doi.org/10.1016/S0021-9673%2801%2984479-4, https://doi.org/10.1016/S0021-9673(01)84479-4