The aim of this study was to measure high-resolution strain fields in planar sections of brain tissueduring translational acceleration to obtain validation data for numerical simulations. Slices were madefrom fresh, porcine brain tissue, and contained both grey and white matter as well as the complexfolding structure of the cortex. The brain slices were immersed in artificial cerebrospinal fluid (aCSF)and were encapsulated in a rigid cavity representing the actual shape of the skull. The rigid cavitysustained an acceleration of about 86 g to a velocity of 4 m/s followed by a deceleration of morethan 200 g. During the experiment, images were taken using a high-speed video camera and VonMises strains were calculated using a digital image correlation technique. The acceleration of thesampleholder was determined using the same digital image correlation technique. A rotational motionof the brain slice relative to the sampleholder was observed, which may have been caused by a thickerposterior part of the slice. Local variations in the displacement field were found, which were relatedto the sulci and the grey and white matter composition of the slice. Furthermore, higher Von Misesstrains were seen in the areas around the sulci.