On the isolation of single basic amino acids with electrodialysis for the production of biochemical

O.M. Kattan Readi, M. Girones, W. Wiratha, K. Nijmeijer

Research output: Contribution to journalArticleAcademicpeer-review

17 Citations (Scopus)

Abstract

Amino acids from biobased feeds are an interesting feedstock for the production of biobased chemicals from cheap protein sources, as amino acids already have the required functionalities. Amino acids are zwitterionic molecules whose charge is determined by the surrounding pH. This makes the use of an electrical field as driving force for their separation, as in membrane electrodialysis (ED), attractive. Electrodialysis with commercially available ion exchange membranes was applied for the isolation of the basic amino acids l-lysine (Lys) and l-arginine (Arg) as a mixture and further separation of Arg from 1,5 pentanediamine (PDA), which is obtained from the enzymatic conversion of Lys. Electrodialysis experiments for the separation of PDA from Arg are carried out at three different pH values: 12.5 (PDA0/Arg–0.5), 10.9 (PDA+0.5/Arg0), and 10.0 (PDA+1.5/Arg0). Owing to the sensitivity of the charge of the amino acids with respect to pH changes, experiments at constant pH (using acid or base dosing or using a buffer in the feed solution) are carried out as well. Control of the pH significantly improves the performance of the process. The separation of PDA from Arg at pH = 10.0, when PDA has an average net charge of +1.5, resulted in the highest recovery (63%) at the highest current efficiency (83%) and significantly low energy consumption (3 kWh/kg). Depending on the conditions, pure streams of amino acids, either Arg (at pH = 12.5) or PDA (pH = 10.9) could be obtained, which shows the strength of the concept of enzymatic conversion combined with electrodialysis for the fractionation of amino acids for biorefinery applications
Original languageEnglish
Pages (from-to)1069
Number of pages1078
JournalIndustrial and Engineering Chemistry Research
Volume52
Issue number3
DOIs
Publication statusPublished - 2013
Externally publishedYes

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Basic Amino Acids
Electrodialysis
Arginine
Amino acids
Amino Acids
Lysine
Cadaverine
Ion exchange membranes
Fractionation
Feedstocks
Buffers
Energy utilization
Experiments
Membranes
Recovery
Molecules
Acids
Proteins

Cite this

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title = "On the isolation of single basic amino acids with electrodialysis for the production of biochemical",
abstract = "Amino acids from biobased feeds are an interesting feedstock for the production of biobased chemicals from cheap protein sources, as amino acids already have the required functionalities. Amino acids are zwitterionic molecules whose charge is determined by the surrounding pH. This makes the use of an electrical field as driving force for their separation, as in membrane electrodialysis (ED), attractive. Electrodialysis with commercially available ion exchange membranes was applied for the isolation of the basic amino acids l-lysine (Lys) and l-arginine (Arg) as a mixture and further separation of Arg from 1,5 pentanediamine (PDA), which is obtained from the enzymatic conversion of Lys. Electrodialysis experiments for the separation of PDA from Arg are carried out at three different pH values: 12.5 (PDA0/Arg–0.5), 10.9 (PDA+0.5/Arg0), and 10.0 (PDA+1.5/Arg0). Owing to the sensitivity of the charge of the amino acids with respect to pH changes, experiments at constant pH (using acid or base dosing or using a buffer in the feed solution) are carried out as well. Control of the pH significantly improves the performance of the process. The separation of PDA from Arg at pH = 10.0, when PDA has an average net charge of +1.5, resulted in the highest recovery (63{\%}) at the highest current efficiency (83{\%}) and significantly low energy consumption (3 kWh/kg). Depending on the conditions, pure streams of amino acids, either Arg (at pH = 12.5) or PDA (pH = 10.9) could be obtained, which shows the strength of the concept of enzymatic conversion combined with electrodialysis for the fractionation of amino acids for biorefinery applications",
author = "{Kattan Readi}, O.M. and M. Girones and W. Wiratha and K. Nijmeijer",
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journal = "Industrial and Engineering Chemistry Research",
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On the isolation of single basic amino acids with electrodialysis for the production of biochemical. / Kattan Readi, O.M.; Girones, M.; Wiratha, W.; Nijmeijer, K.

In: Industrial and Engineering Chemistry Research, Vol. 52, No. 3, 2013, p. 1069.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - On the isolation of single basic amino acids with electrodialysis for the production of biochemical

AU - Kattan Readi, O.M.

AU - Girones, M.

AU - Wiratha, W.

AU - Nijmeijer, K.

PY - 2013

Y1 - 2013

N2 - Amino acids from biobased feeds are an interesting feedstock for the production of biobased chemicals from cheap protein sources, as amino acids already have the required functionalities. Amino acids are zwitterionic molecules whose charge is determined by the surrounding pH. This makes the use of an electrical field as driving force for their separation, as in membrane electrodialysis (ED), attractive. Electrodialysis with commercially available ion exchange membranes was applied for the isolation of the basic amino acids l-lysine (Lys) and l-arginine (Arg) as a mixture and further separation of Arg from 1,5 pentanediamine (PDA), which is obtained from the enzymatic conversion of Lys. Electrodialysis experiments for the separation of PDA from Arg are carried out at three different pH values: 12.5 (PDA0/Arg–0.5), 10.9 (PDA+0.5/Arg0), and 10.0 (PDA+1.5/Arg0). Owing to the sensitivity of the charge of the amino acids with respect to pH changes, experiments at constant pH (using acid or base dosing or using a buffer in the feed solution) are carried out as well. Control of the pH significantly improves the performance of the process. The separation of PDA from Arg at pH = 10.0, when PDA has an average net charge of +1.5, resulted in the highest recovery (63%) at the highest current efficiency (83%) and significantly low energy consumption (3 kWh/kg). Depending on the conditions, pure streams of amino acids, either Arg (at pH = 12.5) or PDA (pH = 10.9) could be obtained, which shows the strength of the concept of enzymatic conversion combined with electrodialysis for the fractionation of amino acids for biorefinery applications

AB - Amino acids from biobased feeds are an interesting feedstock for the production of biobased chemicals from cheap protein sources, as amino acids already have the required functionalities. Amino acids are zwitterionic molecules whose charge is determined by the surrounding pH. This makes the use of an electrical field as driving force for their separation, as in membrane electrodialysis (ED), attractive. Electrodialysis with commercially available ion exchange membranes was applied for the isolation of the basic amino acids l-lysine (Lys) and l-arginine (Arg) as a mixture and further separation of Arg from 1,5 pentanediamine (PDA), which is obtained from the enzymatic conversion of Lys. Electrodialysis experiments for the separation of PDA from Arg are carried out at three different pH values: 12.5 (PDA0/Arg–0.5), 10.9 (PDA+0.5/Arg0), and 10.0 (PDA+1.5/Arg0). Owing to the sensitivity of the charge of the amino acids with respect to pH changes, experiments at constant pH (using acid or base dosing or using a buffer in the feed solution) are carried out as well. Control of the pH significantly improves the performance of the process. The separation of PDA from Arg at pH = 10.0, when PDA has an average net charge of +1.5, resulted in the highest recovery (63%) at the highest current efficiency (83%) and significantly low energy consumption (3 kWh/kg). Depending on the conditions, pure streams of amino acids, either Arg (at pH = 12.5) or PDA (pH = 10.9) could be obtained, which shows the strength of the concept of enzymatic conversion combined with electrodialysis for the fractionation of amino acids for biorefinery applications

U2 - 10.1021/ie202634v

DO - 10.1021/ie202634v

M3 - Article

VL - 52

SP - 1069

JO - Industrial and Engineering Chemistry Research

JF - Industrial and Engineering Chemistry Research

SN - 0888-5885

IS - 3

ER -