On-line impedimetric monitoring of single cell electrical lysis in a microfluidic device

Sertan Sukas; Albert van den Berg; Leon Terstappen; Séverine Le Gac

On-line impedimetric monitoring of single cell electrical lysis in a microfluidic device

Sertan Sukas
, Albert van den Berg
, Leon Terstappen
, Séverine Le Gac

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

We report a microfluidic device for electrical lysis of cells with in situ monitoring of the process using impedance spectroscopy at the single cell level in a flow-through setting. Breast cancer (MCF-7) cells were continuously supplied through the microchannels, where successive separation and real-time detection of cell components were performed. Achieving sensitive electrical characterization of cancer cells would provide new opportunities for single cell analysis.

Original language	English
Title of host publication	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Publisher	Royal Society of Chemistry
Pages	470-471
Number of pages	2
ISBN (Print)	9781713818526
Publication status	Published - 2019
Externally published	Yes
Event	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 https://microtas2019.org/

Conference

Conference	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Abbreviated title	MicroTAS 2019
Country/Territory	Switzerland
City	Basel
Period	27/10/19 → 31/10/19
Internet address	https://microtas2019.org/

Funding

This work was financially supported by NanoNextNL, a micro and nanotechnology consortium of the Government of The Netherlands and 130 partners. S. Sukas was financially supported by EU H2020 Marie Sk?odowska-Curie Actions (IF 2016, grant agreement no: 752516). This work was financially supported by NanoNextNL, a micro and nanotechnology consortium of the Government of The Netherlands and 130 partners. S. Sukas was financially supported by EU H2020 Marie Skłodowska-Curie Actions (IF 2016, grant agreement no: 752516).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Electrical impedance spectroscopy
Electrical lysis
Lab-on-a-chip
Microfluidics
Single cell analysis

Access to Document

http://toc.proceedings.com/56208webtoc.pdf

Cite this

@inproceedings{34cd65c8be1b4e2e9b5622b73344d46c,

title = "On-line impedimetric monitoring of single cell electrical lysis in a microfluidic device",

abstract = "We report a microfluidic device for electrical lysis of cells with in situ monitoring of the process using impedance spectroscopy at the single cell level in a flow-through setting. Breast cancer (MCF-7) cells were continuously supplied through the microchannels, where successive separation and real-time detection of cell components were performed. Achieving sensitive electrical characterization of cancer cells would provide new opportunities for single cell analysis.",

keywords = "Electrical impedance spectroscopy, Electrical lysis, Lab-on-a-chip, Microfluidics, Single cell analysis",

author = "Sertan Sukas and \{van den Berg\}, Albert and Leon Terstappen and \{Le Gac\}, S{\'e}verine",

note = "Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

language = "English",

isbn = "9781713818526",

pages = "470--471",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

publisher = "Royal Society of Chemistry",

address = "United Kingdom",

url = "https://microtas2019.org/",

}

Sukas, S, van den Berg, A, Terstappen, L & Le Gac, S 2019, On-line impedimetric monitoring of single cell electrical lysis in a microfluidic device. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Royal Society of Chemistry, pp. 470-471, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Basel, Switzerland, 27/10/19. <http://toc.proceedings.com/56208webtoc.pdf>

On-line impedimetric monitoring of single cell electrical lysis in a microfluidic device. / Sukas, Sertan; van den Berg, Albert; Terstappen, Leon et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Royal Society of Chemistry, 2019. p. 470-471.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - On-line impedimetric monitoring of single cell electrical lysis in a microfluidic device

AU - Sukas, Sertan

AU - van den Berg, Albert

AU - Terstappen, Leon

AU - Le Gac, Séverine

PY - 2019

Y1 - 2019

N2 - We report a microfluidic device for electrical lysis of cells with in situ monitoring of the process using impedance spectroscopy at the single cell level in a flow-through setting. Breast cancer (MCF-7) cells were continuously supplied through the microchannels, where successive separation and real-time detection of cell components were performed. Achieving sensitive electrical characterization of cancer cells would provide new opportunities for single cell analysis.

AB - We report a microfluidic device for electrical lysis of cells with in situ monitoring of the process using impedance spectroscopy at the single cell level in a flow-through setting. Breast cancer (MCF-7) cells were continuously supplied through the microchannels, where successive separation and real-time detection of cell components were performed. Achieving sensitive electrical characterization of cancer cells would provide new opportunities for single cell analysis.

KW - Electrical impedance spectroscopy

KW - Electrical lysis

KW - Lab-on-a-chip

KW - Microfluidics

KW - Single cell analysis

UR - https://www.scopus.com/pages/publications/85094964966

M3 - Conference contribution

AN - SCOPUS:85094964966

SN - 9781713818526

SP - 470

EP - 471

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

PB - Royal Society of Chemistry

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Y2 - 27 October 2019 through 31 October 2019

ER -

On-line impedimetric monitoring of single cell electrical lysis in a microfluidic device

Abstract

Conference

Funding

UN SDGs

Keywords

Access to Document

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Cite this