Nucleic acid detection using BRET-beacons based on bioluminescent protein-DNA hybrids

W. Engelen, K.M. van de Wiel, L.H.H. Meijer, B. Saha, M. Merkx

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)
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Abstract

Bioluminescent molecular beacons have been developed using a modular design approach that relies on BRET between the bright luciferase NanoLuc and a Cy3 acceptor. While classical molecular beacons are hampered by background fluorescence and scattering, these BRET-beacons allow detection of low pM concentrations of nucleic acids directly in complex media.

Original languageEnglish
Pages (from-to)2862-2865
Number of pages4
JournalChemical Communications
Volume53
Issue number19
DOIs
Publication statusPublished - 7 Mar 2017

Keywords

  • Bioluminescence Resonance Energy Transfer Techniques
  • DNA/analysis
  • Fluorescence
  • Luminescent Proteins/chemistry

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