Noninvasive imaging of bone-specific collagen i expression in a luciferase transgenic mouse model

Jun Liu, Roel Arends, Anton Martens, Maria H.C. Den Brok-Bardoel, Marian G.H. Scheepers, Clemens A. Van Blitterswijk, Jan De Boer

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)


Luciferase transgenic mice are a very promising tool for noninvasive, quantitative, and longitudinal evaluation of gene expression. The aim of this study was to validate the Col(I)-Luc transgenic mouse model in which the luciferase gene is driven by bone-specific regulatory elements from the mouse collagen α1(I) gene for bioluminescent imaging of bone development and remodeling. We observed strong luciferase activity in skeletal tissues of Col(I)-Luc mice, and observed that the light intensity declined with postnatal bone development. Luciferase activity was enhanced in a tail bone repair model and we were able to monitor the process of ectopic bone formation induced by recombinant human bone morphogenetic protein 2 using bioluminescent imaging. We conclude that Col(I)-Luc transgenic mice can be applied in the field of bone tissue engineering for monitoring bone repair processes and for investigating osteoinductive molecules or scaffolds.

Original languageEnglish
Pages (from-to)1297-1304
Number of pages8
JournalTissue Engineering - Part C: Methods
Issue number6
Publication statusPublished - 1 Dec 2010
Externally publishedYes

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