Nanostructuring lipophilic dyes in water using stable vesicles, quatsomes, as scaffolds and their use as probes for bioimaging

Antonio Ardizzone; Siarhei Kurhuzenkau; Sílvia Illa-Tuset; Jordi Faraudo; Mykhailo Bondar; David Hagan; Eric W. Van Stryland; Anna Painelli; Cristina Sissa; Natalia Feiner; Lorenzo Albertazzi; Jaume Veciana; Nora Ventosa

doi:10.1002/smll.201703851

Nanostructuring lipophilic dyes in water using stable vesicles, quatsomes, as scaffolds and their use as probes for bioimaging

Antonio Ardizzone, Siarhei Kurhuzenkau, Sílvia Illa-Tuset, Jordi Faraudo, Mykhailo Bondar, David Hagan, Eric W. Van Stryland, Anna Painelli, Cristina Sissa, Natalia Feiner, Lorenzo Albertazzi, Jaume Veciana, Nora Ventosa

Molecular Biosensing

Research output: Contribution to journal › Article › Academic › peer-review

26 Citations (Scopus)

Abstract

A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate (DiI), 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indodicarbocyanine perchlorate (DiD), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO₂-based technology, show excellent colloidal- and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI-loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.

Original language	English
Article number	1703851
Number of pages	6
Journal	Small : Nano Micro
Volume	14
Issue number	16
DOIs	https://doi.org/10.1002/smll.201703851
Publication status	Published - 19 Apr 2018

Funding

Dr. A. Ardizzone, Dr. J. Faraudo, Prof. J. Veciana, Dr. N. Ventosa Institut Ciencia Materials Barcelona (ICMAB-CSIC)-CIBER-BBN Campus Universitari de Bellaterra 08193 Cerdanyola, Spain E-mail: [email protected]; [email protected] Dr. S. Kurhuzenkau, Prof. A. Painelli, Dr. C. Sissa Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale Università di Parma Parco Area Delle Scienze 17/A, 43124 Parma, Italy S. Illa-Tuset Theoretical Sciences Unit JNCASR Bangalore 560064, India Prof. M. Bondar Institute of Physics National Academy of Sciences of Ukraine Prospect Nauky 46, Kyiv 03028, Ukraine Dr. D. Hagan, Prof. E. W. Van Stryland The College of Optics and Photonics (CREOL) University of Central Florida P.O. Box 162700, Orlando, FL 32816-2700, USA N. Feiner, Dr. L. Albertazzi Institute for Bioengineering of Catalonia (IBEC) Parc Cientìfic de Barcelona (PCB) 08028 Barcelona, Spain The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smll.201703851. This work was financially supported by MINECO/FEDER, Spain (Grant Nos. CTQ2013-40480-R, SAF2016-7524-R, and MAT 2016-80826-R), by AGAUR, Generalitat de Catalunya (Grant No. 2014-SGR-17), and through the CERCA program, the Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN) and the Spanish Ministry of Economy and Competitiveness, through the “Severo Ochoa” Programme for Centres of Excellence in R&D (SEV-2015-0496, Grant FLOWERS, and SEV-2014-0425). L.A. thanks the financial support of AXA Research Fund and D.H. and E.V.S. the Army Research Laboratory (Grant No. W911NF-15-2-0090). The research leading to these results received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework ProgrammeFP7/2007–2013 under REA grant Agreement No. 607721 (Nano2Fun) A.A. and S.I.-T. are enrolled in the Materials Science Ph.D. program of UAB. A.A. and S.I.-T. gratefully acknowledge help of Dr. M. Masino in photostability measurements.

Keywords

dyes
fluorescent nanoparticles
molecular dynamics
STORM
vesicles
Nanostructures/chemistry
Nanoparticles/chemistry
Water/chemistry
Molecular Dynamics Simulation

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10.1002/smll.201703851

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Ardizzone, A., Kurhuzenkau, S., Illa-Tuset, S., Faraudo, J., Bondar, M., Hagan, D., Van Stryland, E. W., Painelli, A., Sissa, C., Feiner, N., Albertazzi, L., Veciana, J., & Ventosa, N. (2018). Nanostructuring lipophilic dyes in water using stable vesicles, quatsomes, as scaffolds and their use as probes for bioimaging. Small : Nano Micro, 14(16), Article 1703851. https://doi.org/10.1002/smll.201703851

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title = "Nanostructuring lipophilic dyes in water using stable vesicles, quatsomes, as scaffolds and their use as probes for bioimaging",

abstract = "A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate (DiI), 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indodicarbocyanine perchlorate (DiD), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO2-based technology, show excellent colloidal- and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI-loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.",

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author = "Antonio Ardizzone and Siarhei Kurhuzenkau and S{\'i}lvia Illa-Tuset and Jordi Faraudo and Mykhailo Bondar and David Hagan and \{Van Stryland\}, \{Eric W.\} and Anna Painelli and Cristina Sissa and Natalia Feiner and Lorenzo Albertazzi and Jaume Veciana and Nora Ventosa",

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Ardizzone, A, Kurhuzenkau, S, Illa-Tuset, S, Faraudo, J, Bondar, M, Hagan, D, Van Stryland, EW, Painelli, A, Sissa, C, Feiner, N, Albertazzi, L, Veciana, J & Ventosa, N 2018, 'Nanostructuring lipophilic dyes in water using stable vesicles, quatsomes, as scaffolds and their use as probes for bioimaging', Small : Nano Micro, vol. 14, no. 16, 1703851. https://doi.org/10.1002/smll.201703851

TY - JOUR

T1 - Nanostructuring lipophilic dyes in water using stable vesicles, quatsomes, as scaffolds and their use as probes for bioimaging

AU - Ardizzone, Antonio

AU - Kurhuzenkau, Siarhei

AU - Illa-Tuset, Sílvia

AU - Faraudo, Jordi

AU - Bondar, Mykhailo

AU - Hagan, David

AU - Van Stryland, Eric W.

AU - Painelli, Anna

AU - Sissa, Cristina

AU - Feiner, Natalia

AU - Albertazzi, Lorenzo

AU - Veciana, Jaume

AU - Ventosa, Nora

PY - 2018/4/19

Y1 - 2018/4/19

N2 - A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate (DiI), 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indodicarbocyanine perchlorate (DiD), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO2-based technology, show excellent colloidal- and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI-loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.

AB - A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate (DiI), 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indodicarbocyanine perchlorate (DiD), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO2-based technology, show excellent colloidal- and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI-loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.

KW - dyes

KW - fluorescent nanoparticles

KW - molecular dynamics

KW - STORM

KW - vesicles

KW - Nanostructures/chemistry

KW - Nanoparticles/chemistry

KW - Water/chemistry

KW - Molecular Dynamics Simulation

UR - https://www.scopus.com/pages/publications/85045852718

U2 - 10.1002/smll.201703851

DO - 10.1002/smll.201703851

M3 - Article

C2 - 29573545

AN - SCOPUS:85045852718

SN - 1613-6810

VL - 14

JO - Small : Nano Micro

JF - Small : Nano Micro

IS - 16

M1 - 1703851

ER -

Nanostructuring lipophilic dyes in water using stable vesicles, quatsomes, as scaffolds and their use as probes for bioimaging

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