Nanoscopy for endosomal escape quantification

Teodora Andrian, Roger Riera, Silvia Pujals, Lorenzo Albertazzi (Corresponding author)

Research output: Contribution to journalReview articlepeer-review

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Abstract

The successful cytosolic delivery of nanoparticles is hampered by their endosomal entrapment and degradation. To push forward the smart development of nanoparticles we must reliably detect and quantify their endosomal escape process. However, the current methods employed are not quantitative enough at the nanoscale to achieve this. Nanoscopy is a rapidly evolving field that has developed a diverse set of powerful techniques in the last two decades, opening the door to explore nanomedicine with an unprecedented resolution and specificity. The understanding of key steps in the drug delivery process-such as endosomal escape-would benefit greatly from the implementation of the most recent advances in microscopy. In this review, we provide the latest insights into endosomal escape of nanoparticles obtained by nanoscopy, and we discuss the features that would allow these techniques to make a great impact in the field.

Original languageEnglish
Pages (from-to)10-23
Number of pages14
JournalNanoscale Advances
Volume3
Issue number1
DOIs
Publication statusPublished - 7 Jan 2021

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