Nanoscaffold's stiffness affects primary cortical cell network formation

S. Xie, B. Schurink, F. Wolbers, R. Luttge, G. Hassink

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Networks of neurons cultured on-chip can provide insights into both normal and disease-state brain function. The ability to guide neuronal growth in specific, artificially designed patterns allows us to study how brain function follows form. Primary cortical cells cultured on nanograting scaffolds, in particular astrocytes, showed highly ordered regions of dendritic outgrowth. Usually, materials suitable for nanopatterning have a stiffness far above that of the extracellular matrix. In this paper, the authors studied two materials with large differences in stiffness, polydimethylsiloxane (PDMS) and silicon. Our results show that both nanopatterned silicon and PDMS guide the outgrowth of astrocytes in cortical cell culture, but the growth of the astrocyte is affected by the stiffness of the substrate, as revealed by differences in the cell soma size and the organization of the outgrowth.

LanguageEnglish
Article number06FD03
Number of pages5
JournalJournal of Vacuum Science and Technology B: Nanotechnology and Microelectronics
Volume32
Issue number6
DOIs
StatePublished - 1 Nov 2014

Fingerprint

stiffness
Stiffness
Silicon
Polydimethylsiloxane
brain
Brain
cells
Forms (concrete)
Carisoprodol
silicon
neurons
Cell culture
cultured cells
Scaffolds
Neurons
chips
Substrates
matrices
Astrocytes
baysilon

Cite this

@article{b3d5228a19504ef8b69f80c7be7398b7,
title = "Nanoscaffold's stiffness affects primary cortical cell network formation",
abstract = "Networks of neurons cultured on-chip can provide insights into both normal and disease-state brain function. The ability to guide neuronal growth in specific, artificially designed patterns allows us to study how brain function follows form. Primary cortical cells cultured on nanograting scaffolds, in particular astrocytes, showed highly ordered regions of dendritic outgrowth. Usually, materials suitable for nanopatterning have a stiffness far above that of the extracellular matrix. In this paper, the authors studied two materials with large differences in stiffness, polydimethylsiloxane (PDMS) and silicon. Our results show that both nanopatterned silicon and PDMS guide the outgrowth of astrocytes in cortical cell culture, but the growth of the astrocyte is affected by the stiffness of the substrate, as revealed by differences in the cell soma size and the organization of the outgrowth.",
author = "S. Xie and B. Schurink and F. Wolbers and R. Luttge and G. Hassink",
year = "2014",
month = "11",
day = "1",
doi = "10.1116/1.4900420",
language = "English",
volume = "32",
journal = "Journal of Vacuum Science and Technology B: Nanotechnology and Microelectronics",
issn = "2166-2746",
publisher = "AVS Science and Technology Society",
number = "6",

}

Nanoscaffold's stiffness affects primary cortical cell network formation. / Xie, S.; Schurink, B.; Wolbers, F.; Luttge, R.; Hassink, G.

In: Journal of Vacuum Science and Technology B: Nanotechnology and Microelectronics, Vol. 32, No. 6, 06FD03, 01.11.2014.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Nanoscaffold's stiffness affects primary cortical cell network formation

AU - Xie,S.

AU - Schurink,B.

AU - Wolbers,F.

AU - Luttge,R.

AU - Hassink,G.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Networks of neurons cultured on-chip can provide insights into both normal and disease-state brain function. The ability to guide neuronal growth in specific, artificially designed patterns allows us to study how brain function follows form. Primary cortical cells cultured on nanograting scaffolds, in particular astrocytes, showed highly ordered regions of dendritic outgrowth. Usually, materials suitable for nanopatterning have a stiffness far above that of the extracellular matrix. In this paper, the authors studied two materials with large differences in stiffness, polydimethylsiloxane (PDMS) and silicon. Our results show that both nanopatterned silicon and PDMS guide the outgrowth of astrocytes in cortical cell culture, but the growth of the astrocyte is affected by the stiffness of the substrate, as revealed by differences in the cell soma size and the organization of the outgrowth.

AB - Networks of neurons cultured on-chip can provide insights into both normal and disease-state brain function. The ability to guide neuronal growth in specific, artificially designed patterns allows us to study how brain function follows form. Primary cortical cells cultured on nanograting scaffolds, in particular astrocytes, showed highly ordered regions of dendritic outgrowth. Usually, materials suitable for nanopatterning have a stiffness far above that of the extracellular matrix. In this paper, the authors studied two materials with large differences in stiffness, polydimethylsiloxane (PDMS) and silicon. Our results show that both nanopatterned silicon and PDMS guide the outgrowth of astrocytes in cortical cell culture, but the growth of the astrocyte is affected by the stiffness of the substrate, as revealed by differences in the cell soma size and the organization of the outgrowth.

UR - http://www.scopus.com/inward/record.url?scp=84949123402&partnerID=8YFLogxK

U2 - 10.1116/1.4900420

DO - 10.1116/1.4900420

M3 - Article

VL - 32

JO - Journal of Vacuum Science and Technology B: Nanotechnology and Microelectronics

T2 - Journal of Vacuum Science and Technology B: Nanotechnology and Microelectronics

JF - Journal of Vacuum Science and Technology B: Nanotechnology and Microelectronics

SN - 2166-2746

IS - 6

M1 - 06FD03

ER -