Myofibrillar distribution of succinate dehydrogenase activity and lipid stores differs in skeletal muscle tissue of paraplegic subjects

R.A.M. Jonkers, M.L. Dirks, C.I.H.C. Nabuurs, H.M.M.L. Feyter, de, S.F.E. Praet, K. Nicolay, L.J.C. Loon, van, J.J. Prompers

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Lack of physical activity has been related to an increased risk of developing insulin resistance. This study aimed to assess the impact of chronic muscle deconditioning on whole body insulin sensitivity, muscle oxidative capacity, and intramyocellular lipid (IMCL) content in subjects with paraplegia. Nine subjects with paraplegia and nine able-bodied, lean controls were recruited. An oral glucose tolerance test was performed to assess whole body insulin sensitivity. IMCL content was determined both in vivo and in vitro using 1H-magnetic resonance spectroscopy and fluorescence microscopy, respectively. Muscle biopsy samples were stained for succinate dehydrogenase (SDH) activity to measure muscle fiber oxidative capacity. Subcellular distributions of IMCL and SDH activity were determined by defining subsarcolemmal and intermyofibrillar areas on histological samples. SDH activity was 57 ± 14% lower in muscle fibers derived from subjects with paraplegia when compared with controls (P < 0.05),
Original languageEnglish
Pages (from-to)365-373
JournalAmerican Journal of Physiology : Endocrinology and Metabolism
Volume302
Issue number3
DOIs
Publication statusPublished - 2012

Fingerprint Dive into the research topics of 'Myofibrillar distribution of succinate dehydrogenase activity and lipid stores differs in skeletal muscle tissue of paraplegic subjects'. Together they form a unique fingerprint.

Cite this