Multivalent choline dendrimers increase phagocytosis of streptococcus pneumoniae R6 by microglial cells

S. Ribes, J. Riegelmann, S. Redlich, B. Maestro, B.F.M. Waal, de, E.W. Meijer, J.M. Sanz, R. Nau

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)


Background: Pneumococcal virulence factors common to all serotypes, such as choline-binding proteins (CBPs), are promising therapeutic targets in pneumococcal infections. We studied the effect of a choline dendrimer with maximized binding affinity/specificity for CBPs on microglia-mediated pneumococcal phagocytosis. Methods: Pneumoccocal cultures were exposed to dendrinners containing 8 choline end groups or amino groups as controls, either from the beginning of bacterial growth or at the late exponential phase. The effect of long/short co-incubation was assessed in terms of bacterial morphological changes and increase in bacterial uptake by primary microglial cultures. Results: Inhibiting CBPs by micronnolar concentrations of a choline dendrimer caused the formation of long pneumococcal chains that were readily phagocytosed by microglia. Enhanced phagocytosis was dendrimer dose-dependent. Long bacteria-dendrimer co-incubation (14 h) resulted in a higher bacterial uptake than short co-incubation (2 h; p <0.001). Conclusions: Multivalent dendrinners containing choline end groups are promising antimicrobial agents for the management of pneumococcal diseases. (C) 2013 S.Karger AG, Basel
Original languageEnglish
Pages (from-to)138-142
Number of pages5
Issue number2
Publication statusPublished - Oct 2013


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