MRI of ICAM-1 upregulation after stroke : the importance of choosing the appropriate target-specific particulate contrast agent

L.H. Deddens, G.A.F. Tilborg, van, A. Toorn, van der, K. Marel, van der, L.E.M. Paulis, L. Bloois, van, G. Storm, G.J. Strijkers, W.J.M. Mulder, H.E. Vries, de, R.M. Dijkhuizen

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    33 Citations (Scopus)

    Abstract

    Magnetic resonance imaging (MRI) with targeted contrast agents provides a promising means for diagnosis and treatment monitoring after cerebrovascular injury. Our goal was to demonstrate the feasibility of this approach to detect the neuroinflammatory biomarker intercellular adhesion molecule-1 (ICAM-1) after stroke and to establish a most efficient imaging procedure. We compared two types of ICAM-1-functionalized contrast agent: T (1)-shortening gadolinium chelate-containing liposomes and T (2) (()*())-shortening micron-sized iron oxide particles (MPIO). Binding efficacy and MRI contrast effects were tested in cell cultures and a mouse stroke model. Both ICAM-1-targeted agents bound effectively to activated cerebrovascular cells in vitro, generating significant MRI contrast-enhancing effects. Direct in vivo MRI-based detection after stroke was only achieved with ICAM-1-targeted MPIO, although both contrast agents showed similar target-specific vascular accumulation. Our study demonstrates the potential of in vivo MRI of post-stroke ICAM-1 upregulation and signifies target-specific MPIO as most suitable contrast agent for molecular MRI of cerebrovascular inflammation.
    Original languageEnglish
    Pages (from-to)411-422
    Number of pages12
    JournalMolecular Imaging and Biology
    Volume15
    Issue number4
    DOIs
    Publication statusPublished - 2013

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