Moving boundary electrophoresis is a collective noun for various electrophoretic procedures, and can be performed in various ways. Commonly, the species to be separated determine whether a background conductance of ionic species is wanted. Moving boundary electrophoresis has been especially applied in the separation of high-molecular-weight substances. The choice of the buffers is normally determined by whether a separation of anions or cations is required. An unavoidable discontinuity in the conducting circuit is because of the boundary between the sample to be separated and the electrode solutions. As soon as the electric current is switched on, the sample ions migrate in the appropriate direction and form separating boundaries both at the front side and at the rear. When carrying out experiments on moving boundary electrophoresis, the narrow-bore tube can be filled with an electrolyte of a strong acid if the separation of, for example, cations is desired. The cation present has a mobility that is higher than that of any other cation in the sample.
|Name||Journal of Chromatography Library|