Plasmodium knowlesi infection with low parasitemia presents a diagnostic challenge, as rapid diagnostic tests are often negative and identification to the species level by microscopy is difficult. P. knowlesi malaria in a traveler is described, and real-time PCR is demonstrated to support fast and reliable diagnosis and identification to the species level.
Link, L., Bart, A., Verhaar, N., Gool, van, T., Pronk, M., & Scharnhorst, V. (2014). Molecular detection of Plasmodium knowlesi in a Dutch traveller by real-time PCR. Journal of Clinical Microbiology, 50(7), 2523-2524. https://doi.org/10.1128/JCM.06859-11