Modification, permethylation, and mass spectrometry of arginine-containing oligopeptides at the 100 nanomolar level

P.A. Leclercq, L.C. Smith, D.M. Desiderio

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

The amino acid sequence of arginine-contg. oligopeptides, including bradykinin, was detd. by mass spectrometry. The guanidino groups of the arginine residues in 100 nmoles of peptide were reacted with acetyl-acetone under mild conditions to produce Nd-2-(4,6-dimethyl)pyrimidylornithine residues. N-Acettylation and N,O-permethylation then produced peptide derivs. that were suitable for vaporization. This procedure makes arginine-contg. oligopeptides accessible for mass spectrometric sequence anal. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)937-944
JournalBiochemical and Biophysical Research Communications
Volume45
Issue number4
DOIs
Publication statusPublished - 1971

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