Modeling of enzymatic transesterification for omega-3 fatty acids enrichment in fish oil

Michele Ongis; D. Liese; Gioele Di Marcoberardino; F. Gallucci; M. Binotti

doi:10.1016/j.foodchem.2024.141379

Modeling of enzymatic transesterification for omega-3 fatty acids enrichment in fish oil

Michele Ongis (Corresponding author), D. Liese, Gioele Di Marcoberardino, F. Gallucci, M. Binotti (Corresponding author)

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Abstract

Mathematical models of transesterification commonly assume that oil is a mixture of triacylglycerols, where each component has only one type of acid attached. This article aims to show how a different assumption on acid distribution affects the results of acylglycerols fraction composition. Experiments of fish oil ethanolysis have been performed at different enzyme loadings and ethanol concentrations, leading to enrichments from 35 % to 52 % of ω3 mass fraction in acylglycerols, by losing 12.1 % of ω3 as ethyl esters. A kinetic model is developed assuming both all acids of the same type on each acylglycerol and all acids randomly distributed on the available positions. The two different assumptions showed strong discrepancies on the acylglycerols fraction compositions predictions, demonstrating how the initial fatty acids distribution is important when an accurate description of the acylglycerols fraction is desired.

Original language	English
Article number	141379
Number of pages	14
Journal	Food Chemistry
Volume	463
Issue number	Part 3.
Early online date	20 Sept 2024
DOIs	https://doi.org/10.1016/j.foodchem.2024.141379
Publication status	Published - 15 Jan 2025

Funding

This project has received funding from the European Union's Horizon 2020 Research and Innovation Program under grant agreement No. 869896 (MACBETH) . [Formula presented]This project has received funding from the European Union's Horizon 2020 Research and Innovation Program under grant agreement No. 869896 (MACBETH). ChiralVision B.V. is acknowledged for the enzyme formulation and immobilization on the beads. Solutex Corp. is acknowledged for supply of the fish oil.

Funders	Funder number
European Union's Horizon 2020 - Research and Innovation Framework Programme	869896

Keywords

Fatty acid enrichment
Fish oil
Kinetics
Modeling
Transesterification

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10.1016/j.foodchem.2024.141379

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abstract = "Mathematical models of transesterification commonly assume that oil is a mixture of triacylglycerols, where each component has only one type of acid attached. This article aims to show how a different assumption on acid distribution affects the results of acylglycerols fraction composition. Experiments of fish oil ethanolysis have been performed at different enzyme loadings and ethanol concentrations, leading to enrichments from 35 % to 52 % of ω3 mass fraction in acylglycerols, by losing 12.1 % of ω3 as ethyl esters. A kinetic model is developed assuming both all acids of the same type on each acylglycerol and all acids randomly distributed on the available positions. The two different assumptions showed strong discrepancies on the acylglycerols fraction compositions predictions, demonstrating how the initial fatty acids distribution is important when an accurate description of the acylglycerols fraction is desired.",

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AU - Binotti, M.

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AB - Mathematical models of transesterification commonly assume that oil is a mixture of triacylglycerols, where each component has only one type of acid attached. This article aims to show how a different assumption on acid distribution affects the results of acylglycerols fraction composition. Experiments of fish oil ethanolysis have been performed at different enzyme loadings and ethanol concentrations, leading to enrichments from 35 % to 52 % of ω3 mass fraction in acylglycerols, by losing 12.1 % of ω3 as ethyl esters. A kinetic model is developed assuming both all acids of the same type on each acylglycerol and all acids randomly distributed on the available positions. The two different assumptions showed strong discrepancies on the acylglycerols fraction compositions predictions, demonstrating how the initial fatty acids distribution is important when an accurate description of the acylglycerols fraction is desired.

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Modeling of enzymatic transesterification for omega-3 fatty acids enrichment in fish oil

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