Abstract
We investigate a fast and sensitive optomagnetic bionanotechnology for biomarker detection. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. These particle clusters can be analyzed using a rotating magnetic field which induces an oscillating light scattering cross-section, resolving sub-picomolar biomarker concentrations. [1,2]
In this paper we describe model assays to control the particle-particle distance. These will be used to verify if this distance can be determined from the scattering signals, as is predicted by multi-particle Mie scattering simulations. The first assay that has been studied consists of protein G coated magnetic particles, which bind anti-biotin IgG and are then linked by biotin-coated fluorescent particles. The IgG and fluorescent particle concentrations are varied and the formed clusters are studied using a FACS, which allows distinction of cluster sizes and the presence of fluorescent particles. After sorting the fluorescent doublets, the particles in the doublets redistribute. As a consequence, the resulting cluster distribution is not suited to study the scattering signals. An alternative model assay is presently investigated and consists of amine-modified fluorescent particles and carboxylated magnetic particles with the purpose to form covalent bonds preventing redistribution after sorting.
1: Ranzoni et al, Nano Letters 2011 11 (5), 2017-2022
2: Ranzoni et al, ACS Nano 2012 6 (4), 3134-3141
| Original language | English |
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| Publication status | Published - 2014 |
| Event | conference; Dutch meeting on molecular and cellular biophysics, 2014; 2014-09-29; 2014-09-30 - Duration: 29 Sept 2014 → 30 Sept 2014 |
Conference
| Conference | conference; Dutch meeting on molecular and cellular biophysics, 2014; 2014-09-29; 2014-09-30 |
|---|---|
| Period | 29/09/14 → 30/09/14 |
| Other | Dutch meeting on molecular and cellular biophysics, 2014 |