Model assays to vary particle-particle distances in an optomagnetic cluster assay

R.W.L. Vliembergen, van, L.J. IJzendoorn, van, M.W.J. Prins

Research output: Contribution to conferencePosterAcademic

Abstract

We investigate a fast and sensitive optomagnetic bionanotechnology for biomarker detection. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. These particle clusters can be analyzed using a rotating magnetic field which induces an oscillating light scattering cross-section, resolving sub-picomolar biomarker concentrations. [1,2] In this paper we describe model assays to control the particle-particle distance. These will be used to verify if this distance can be determined from the scattering signals, as is predicted by multi-particle Mie scattering simulations. The first assay that has been studied consists of protein G coated magnetic particles, which bind anti-biotin IgG and are then linked by biotin-coated fluorescent particles. The IgG and fluorescent particle concentrations are varied and the formed clusters are studied using a FACS, which allows distinction of cluster sizes and the presence of fluorescent particles. After sorting the fluorescent doublets, the particles in the doublets redistribute. As a consequence, the resulting cluster distribution is not suited to study the scattering signals. An alternative model assay is presently investigated and consists of amine-modified fluorescent particles and carboxylated magnetic particles with the purpose to form covalent bonds preventing redistribution after sorting. 1: Ranzoni et al, Nano Letters 2011 11 (5), 2017-2022 2: Ranzoni et al, ACS Nano 2012 6 (4), 3134-3141
Original languageEnglish
Publication statusPublished - 2014
Eventconference; Dutch meeting on molecular and cellular biophysics, 2014; 2014-09-29; 2014-09-30 -
Duration: 29 Sep 201430 Sep 2014

Conference

Conferenceconference; Dutch meeting on molecular and cellular biophysics, 2014; 2014-09-29; 2014-09-30
Period29/09/1430/09/14
OtherDutch meeting on molecular and cellular biophysics, 2014

Bibliographical note

Proceedings of the Dutch Meeting on Molecular and Cellular Biophysics, 29-30 September 2014, Veldhoven, The Netherlands

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    Vliembergen, van, R. W. L., IJzendoorn, van, L. J., & Prins, M. W. J. (2014). Model assays to vary particle-particle distances in an optomagnetic cluster assay. Poster session presented at conference; Dutch meeting on molecular and cellular biophysics, 2014; 2014-09-29; 2014-09-30, .