Microfluidics for cell-based high throughput screening platforms : a review

Guansheng Du, Q. Fang, Jaap den Toonder

Research output: Contribution to journalArticleAcademicpeer-review

129 Citations (Scopus)
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Abstract

In the last decades, the basic techniques of microfluidics for the study of cells such as cell culture, cell separation, and cell lysis, have been well developed. Based on cell handling techniques, microfluidics has been widely applied in the field of PCR (Polymerase Chain Reaction), immunoassays, organ-on-chip, stem cell research, and analysis and identification of circulating tumor cells. As a major step in drug discovery, high-throughput screening allows rapid analysis of thousands of chemical, biochemical, genetic or pharmacological tests in parallel. In this review, we summarize the application of microfluidics in cell based high throughput screening. The screening methods mentioned in this paper include approaches using the perfusion flow mode, the droplet mode, and the microarray mode. We also discuss the future development of microfluidic based high throughput screening platform for drug discovery.
Original languageEnglish
Pages (from-to)36-50
JournalAnalytica Chimica Acta
Volume903
DOIs
Publication statusPublished - 22 Nov 2015

Keywords

  • High throughput screening
  • High thCell-based microfluidics
  • Drug discovery

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