Micellar gradients in size-exclusion simulated moving bed chromatography

D.A. Horneman, M. Ottens, J.T.F. Keurentjes, L.A.M. Wielen, van der

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    The selectivity of size-exclusion chromatog. (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatog. (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatog. with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatog. protein sepn. problem.
    Original languageEnglish
    Pages (from-to)130-139
    Number of pages10
    JournalJournal of Chromatography, A
    Issue number1-2
    Publication statusPublished - 2006


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