TY - JOUR
T1 - Micellar gradients in size-exclusion simulated moving bed chromatography
AU - Horneman, D.A.
AU - Ottens, M.
AU - Keurentjes, J.T.F.
AU - Wielen, van der, L.A.M.
PY - 2006
Y1 - 2006
N2 - The selectivity of size-exclusion chromatog. (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatog. (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatog. with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatog. protein sepn. problem.
AB - The selectivity of size-exclusion chromatog. (SEC) can be modified by adding non-ionic micelles to the mobile phase. Surfactant-aided size-exclusion chromatog. (SASEC) can therefore very well be performed in a gradient mode on an SMB, as is reported in this paper. A method has been developed for correctly positioning a micellar gradient over an SMB. The method is applied for size-exclusion chromatog. with the non-ionic surfactant C12E23 as gradient forming solute, and demonstrated by applying it to a relevant chromatog. protein sepn. problem.
U2 - 10.1016/j.chroma.2006.01.134
DO - 10.1016/j.chroma.2006.01.134
M3 - Article
C2 - 16504200
SN - 0021-9673
VL - 1113
SP - 130
EP - 139
JO - Journal of Chromatography, A
JF - Journal of Chromatography, A
IS - 1-2
ER -