Metal ion-induced self-assembly and packaging of CCMV nanocapsules

Lise Schoonen, Mark B. van Eldijk, Jan C.M. van Hest

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

The controlled self-assembly of protein cages is vital for the use of these nanocompartments in biomedical and nanotechnological applications. Recently, we showed that by combining different structural peptide elements, it is possible to assemble viral capsid proteins in distinct well-defined morphologies. In this chapter, a triblock copolypeptide is discussed, consisting of a metal ion-coordinating hexahistidine tag, a stimulus-responsive elastin-like polypeptide and a pH-responsive self-assembling viral capsid protein. This protein is able to form two different types of capsids, depending on the assembly pathway that is followed. Here, we focus on the metal ion-induced assembly process and describe the relevant experimental procedures to induce and utilize this assembly behavior.

LanguageEnglish
Title of host publicationProtein scaffolds
Subtitle of host publicationdesign, synthesis, and applications
EditorsA.K. Udit
Place of PublicationDordrecht
PublisherSpringer
Pages57-67
Number of pages11
ISBN (Electronic)978-1-4939-7893-9
ISBN (Print)978-1-4939-7892-2
DOIs
StatePublished - 5 Jun 2018

Publication series

NameMethods in Molecular Biology
Volume1798
ISSN (Print)1064-3745

Fingerprint

Nanocapsules
His-His-His-His-His-His
Capsid Proteins
Viral Proteins
Product Packaging
Metals
Ions
Peptides
Elastin
Capsid
Proteins

Keywords

  • Affinity chromatography
  • Cowpea chlorotic mottle virus
  • Elastin-like polypeptide
  • Hexahistidine
  • His-tag
  • Metal ion
  • Self-assembly
  • Viral capsid

Cite this

Schoonen, L., van Eldijk, M. B., & van Hest, J. C. M. (2018). Metal ion-induced self-assembly and packaging of CCMV nanocapsules. In A. K. Udit (Ed.), Protein scaffolds: design, synthesis, and applications (pp. 57-67). (Methods in Molecular Biology; Vol. 1798). Dordrecht: Springer. DOI: 10.1007/978-1-4939-7893-9_5
Schoonen, Lise ; van Eldijk, Mark B. ; van Hest, Jan C.M./ Metal ion-induced self-assembly and packaging of CCMV nanocapsules. Protein scaffolds: design, synthesis, and applications. editor / A.K. Udit. Dordrecht : Springer, 2018. pp. 57-67 (Methods in Molecular Biology).
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Schoonen, L, van Eldijk, MB & van Hest, JCM 2018, Metal ion-induced self-assembly and packaging of CCMV nanocapsules. in AK Udit (ed.), Protein scaffolds: design, synthesis, and applications. Methods in Molecular Biology, vol. 1798, Springer, Dordrecht, pp. 57-67. DOI: 10.1007/978-1-4939-7893-9_5

Metal ion-induced self-assembly and packaging of CCMV nanocapsules. / Schoonen, Lise; van Eldijk, Mark B.; van Hest, Jan C.M.

Protein scaffolds: design, synthesis, and applications. ed. / A.K. Udit. Dordrecht : Springer, 2018. p. 57-67 (Methods in Molecular Biology; Vol. 1798).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

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Schoonen L, van Eldijk MB, van Hest JCM. Metal ion-induced self-assembly and packaging of CCMV nanocapsules. In Udit AK, editor, Protein scaffolds: design, synthesis, and applications. Dordrecht: Springer. 2018. p. 57-67. (Methods in Molecular Biology). Available from, DOI: 10.1007/978-1-4939-7893-9_5