Metal-free and pH-controlled introduction of azides in proteins

S. Schoffelen, M.B. van Eldijk, Bart Rooijakkers, Reinout Raijmakers, A.J.R. Heck, J.C.M. van Hest

Research output: Contribution to journalArticleAcademicpeer-review

67 Citations (Scopus)
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Conversion of amines into azides using imidazole-1-sulfonyl azide as a diazotransfer reagent has proven to be a straightforward way to introduce targetable handles into proteins. We explored whether less toxic and milder conditions than described before could be applied. It was shown that the aqueous diazotransfer proceeds without adding Cu(II) not only at pH 11 but also at pH 8.5. The diazotransfer was shown to be selective towards a single amine.

Original languageEnglish
Pages (from-to)701-705
Number of pages5
JournalChemical Science
Issue number4
Publication statusPublished - 2011
Externally publishedYes


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