Metal-free and pH-controlled introduction of azides in proteins

S. Schoffelen; M.B. van Eldijk; Bart Rooijakkers; Reinout Raijmakers; A.J.R. Heck; J.C.M. van Hest

doi:10.1039/c0sc00562b

Metal-free and pH-controlled introduction of azides in proteins

S. Schoffelen, M.B. van Eldijk, Bart Rooijakkers, Reinout Raijmakers, A.J.R. Heck, J.C.M. van Hest

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Abstract

Conversion of amines into azides using imidazole-1-sulfonyl azide as a diazotransfer reagent has proven to be a straightforward way to introduce targetable handles into proteins. We explored whether less toxic and milder conditions than described before could be applied. It was shown that the aqueous diazotransfer proceeds without adding Cu(II) not only at pH 11 but also at pH 8.5. The diazotransfer was shown to be selective towards a single amine.

Original language	English
Pages (from-to)	701-705
Number of pages	5
Journal	Chemical Science
Volume	2
Issue number	4
DOIs	https://doi.org/10.1039/c0sc00562b
Publication status	Published - 2011
Externally published	Yes

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AU - Heck, A.J.R.

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Metal-free and pH-controlled introduction of azides in proteins

Abstract

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