Abstract
We showed for the first time that MEMS actuation promotes in vitro Brain-on-Chip maturation for SH-SY5Y neuroblastoma cells and their networks. Utilizing a repetitive training program and subsequent calcium imaging (CI), we observed increased cellular electrophysiological activity with a trend for increased connectivity at a lower synaptic density, indicating a more matured network upon stimulation.
| Original language | English |
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| Title of host publication | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Royal Society of Chemistry |
| Pages | 957-958 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419017 |
| Publication status | Published - 2020 |
| Event | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online Duration: 4 Oct 2020 → 9 Oct 2020 |
Conference
| Conference | 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 |
|---|---|
| City | Virtual, Online |
| Period | 4/10/20 → 9/10/20 |
Funding
Financial support: ERC-STG (grant no. 476 280281), the Horizon 2020-FET Proactive (grant no. 824070), Health Holland (grant no. LSHM19006). We thank C. Zonnenberg and E. Deniz for the design and realization of the gas-flow controller as well as C. Bouten and M. Chiappalone for inputs to the discussion.
Keywords
- Brain-on-Chip
- Calcium imaging
- Mechanical stimulation
- Mechanotransduction
- SH-SY5Y