MECCSIM, training software for micellar electrokinetic capillary chromatography

J.C. Reijenga, M. Hutta

Research output: Contribution to journalArticleAcademicpeer-review

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171 Downloads (Pure)

Abstract

A training simulation program for micellar electrokinetic capillary chromatography was developed. The software provides a high speed of calculation, visual detail and model accuracy with a user-friendly interface. The instrument parameters that can be varied include capillary material, length, internal diameter and wall thickness, thermostating temperature, voltage and polarity. Hydrostatic injection is simulated using time and pressure as variables. The following properties of the buffer can be varied: pH, ionic strength, an sodium dodecylsulfate (SDS) concentration. The retention model is based on experimental values for dH° and dS°, obtained from the literature. In addition, the temperature and ionic strength dependence of the critical micelle concentration and the partial specific volume of the SDS micelle is taken into account. This allows the calculation of the temperature dependence of the partition coefficients and capacity factors and thus of the retention and migration behaviour. In addition, a number of dispersion factors are included in the model.
Original languageEnglish
Pages (from-to)21-29
Number of pages9
JournalJournal of Chromatography, A
Volume709
DOIs
Publication statusPublished - 1995

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