Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images

M. Veta; A. Huisman; M.A. Viergever; P.J. Diest, van; J.P.W. Pluim

doi:10.1109/ISBI.2011.5872483

Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images

M. Veta, A. Huisman, M.A. Viergever, P.J. Diest, van, J.P.W. Pluim

Medical Image Analysis

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

In this paper we present an unsupervised automatic method for segmentation of nuclei in H&E stained breast cancer biopsy images. Colour deconvolution and morphological operations are used to preprocess the images in order to remove irrelevant structures. Candidate nuclei locations, obtained with the fast radial symmetry transform, act as markers for a marker-controlled watershed segmentation. Watershed regions that are unlikely to represent nuclei are removed in the postprocessing stage. The proposed algorithm is evaluated on a number of images that are representative of the diversity in pathology in this type of tissue. The method shows good performance in terms of the number of segmented nuclei and segmentation accuracy. © 2011 IEEE.

Original language	English
Title of host publication	Proceedings of the 8th IEEE International Symposium on Biomedical Imaging : From Nano to Macro ( ISBI'11), 30 March 2011 through 2 April 2011, Chicago, IL
Place of Publication	Piscataway
Publisher	Institute of Electrical and Electronics Engineers
Pages	618-621
ISBN (Print)	978-1-4244-4128-0
DOIs	https://doi.org/10.1109/ISBI.2011.5872483
Publication status	Published - 2011

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Veta, M., Huisman, A., Viergever, M. A., Diest, van, P. J., & Pluim, J. P. W. (2011). Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images. In Proceedings of the 8th IEEE International Symposium on Biomedical Imaging : From Nano to Macro ( ISBI'11), 30 March 2011 through 2 April 2011, Chicago, IL (pp. 618-621). Institute of Electrical and Electronics Engineers. https://doi.org/10.1109/ISBI.2011.5872483

Veta, M. ; Huisman, A. ; Viergever, M.A. ; Diest, van, P.J. ; Pluim, J.P.W. / Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images. Proceedings of the 8th IEEE International Symposium on Biomedical Imaging : From Nano to Macro ( ISBI'11), 30 March 2011 through 2 April 2011, Chicago, IL. Piscataway : Institute of Electrical and Electronics Engineers, 2011. pp. 618-621

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title = "Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images",

abstract = "In this paper we present an unsupervised automatic method for segmentation of nuclei in H&E stained breast cancer biopsy images. Colour deconvolution and morphological operations are used to preprocess the images in order to remove irrelevant structures. Candidate nuclei locations, obtained with the fast radial symmetry transform, act as markers for a marker-controlled watershed segmentation. Watershed regions that are unlikely to represent nuclei are removed in the postprocessing stage. The proposed algorithm is evaluated on a number of images that are representative of the diversity in pathology in this type of tissue. The method shows good performance in terms of the number of segmented nuclei and segmentation accuracy. {\textcopyright} 2011 IEEE.",

author = "M. Veta and A. Huisman and M.A. Viergever and {Diest, van}, P.J. and J.P.W. Pluim",

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doi = "10.1109/ISBI.2011.5872483",

language = "English",

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publisher = "Institute of Electrical and Electronics Engineers",

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Veta, M, Huisman, A, Viergever, MA, Diest, van, PJ & Pluim, JPW 2011, Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images. in Proceedings of the 8th IEEE International Symposium on Biomedical Imaging : From Nano to Macro ( ISBI'11), 30 March 2011 through 2 April 2011, Chicago, IL. Institute of Electrical and Electronics Engineers, Piscataway, pp. 618-621. https://doi.org/10.1109/ISBI.2011.5872483

Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images. / Veta, M.; Huisman, A.; Viergever, M.A.; Diest, van, P.J.; Pluim, J.P.W.

Proceedings of the 8th IEEE International Symposium on Biomedical Imaging : From Nano to Macro ( ISBI'11), 30 March 2011 through 2 April 2011, Chicago, IL. Piscataway : Institute of Electrical and Electronics Engineers, 2011. p. 618-621.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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AU - Pluim, J.P.W.

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AB - In this paper we present an unsupervised automatic method for segmentation of nuclei in H&E stained breast cancer biopsy images. Colour deconvolution and morphological operations are used to preprocess the images in order to remove irrelevant structures. Candidate nuclei locations, obtained with the fast radial symmetry transform, act as markers for a marker-controlled watershed segmentation. Watershed regions that are unlikely to represent nuclei are removed in the postprocessing stage. The proposed algorithm is evaluated on a number of images that are representative of the diversity in pathology in this type of tissue. The method shows good performance in terms of the number of segmented nuclei and segmentation accuracy. © 2011 IEEE.

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SN - 978-1-4244-4128-0

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BT - Proceedings of the 8th IEEE International Symposium on Biomedical Imaging : From Nano to Macro ( ISBI'11), 30 March 2011 through 2 April 2011, Chicago, IL

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Veta M, Huisman A, Viergever MA, Diest, van PJ, Pluim JPW. Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images. In Proceedings of the 8th IEEE International Symposium on Biomedical Imaging : From Nano to Macro ( ISBI'11), 30 March 2011 through 2 April 2011, Chicago, IL. Piscataway: Institute of Electrical and Electronics Engineers. 2011. p. 618-621 https://doi.org/10.1109/ISBI.2011.5872483

Marker-controlled watershed segmentation of nuclei in H&E stained breast cancer biopsy images

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