"Lyophilisomes": A new type of (bio)capsule

W.F. Daamen, P.J. Geutjes, H.T.B. van Moerkerk, S.T.M. Nillesen, R.G. Wismans, T. Hafmans, L.P.W.J. van den Heuvel, A.M.A. Pistorius, J.H. Veerkamp, T.H. van Kuppevelt, J.C.M. van Hest

Research output: Contribution to journalArticleAcademicpeer-review

39 Citations (Scopus)


A new methodology to prepare lyophilisomes (self-assembled vesicles composed of phospholipids), which are known as biocapsules, from a range of biomolecules was investigated. The samples used included elastin, acetic acid and liquid nitrogen. The technique involved freezing, annealing, and lyophilization procedure. The transmission electron microscopy (TEM) unveiled the capsule nature of the spheres. The walls of the capsules were found to be smooth with equal distribution of protein over it. Fast-freezing in liquid N2 initiated a micro-phase separation between the bulk of the water and an elastin/acetic acid/water compartment. The transition of the spheres into capsules took place due to coffee-stain mechanism, which explains the formation of solid, ringlike stains from solution droplets. The technique offers enough flexibility and is useful for the development of new biocapsules.

Original languageEnglish
Pages (from-to)673-677
Number of pages5
JournalAdvanced Materials
Issue number5
Publication statusPublished - 5 Mar 2007
Externally publishedYes


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