Light-Responsive Elastin-Like Peptide-Based Targeted Nanoparticles for Enhanced Spheroid Penetration

Duc H.T. Le; Vusala Ibrahimova; Sebastian A.H. van den Wildenberg; Hanglong Wu; Alba Fonseca; Tomas Torres; Elisabeth Garanger; William P.J. Leenders; Roland Brock; Sébastien Lecommandoux; Jan C.M. van Hest

doi:10.1002/anie.202300511

Light-Responsive Elastin-Like Peptide-Based Targeted Nanoparticles for Enhanced Spheroid Penetration

Duc H.T. Le
, Vusala Ibrahimova
, Sebastian A.H. van den Wildenberg
, Hanglong Wu
, Alba Fonseca
, Tomas Torres
, Elisabeth Garanger
, William P.J. Leenders
, Roland Brock
, Sébastien Lecommandoux
, Jan C.M. van Hest (Corresponding author)

Research output: Contribution to journal › Article › Academic › peer-review

34 Citations (Scopus)

186 Downloads (Pure)

Abstract

We describe here a near infrared light-responsive elastin-like peptide (ELP)-based targeted nanoparticle (NP) that can rapidly switch its size from 120 to 25 nm upon photo-irradiation. Interestingly, the targeting function, which is crucial for effective cargo delivery, is preserved after transformation. The NPs are assembled from (targeted) diblock ELP micelles encapsulating photosensitizer TT1-monoblock ELP conjugates. Methionine residues in this monoblock are photo-oxidized by singlet oxygen generated from TT1, turning the ELPs hydrophilic and thus trigger NP dissociation. Phenylalanine residues from the diblocks then interact with TT1 via π-π stacking, inducing the re-formation of smaller NPs. Due to their small size and targeting function, the NPs penetrate deeper in spheroids and kill cancer cells more efficiently compared to the larger ones. This work could contribute to the design of “smart” nanomedicines with deeper penetration capacity for effective anticancer therapies.

Original language	English
Article number	e202300511
Number of pages	7
Journal	Angewandte Chemie - International Edition
Volume	62
Issue number	24
DOIs	https://doi.org/10.1002/anie.202300511
Publication status	Published - 12 Jun 2023

Bibliographical note

Funding Information:
This work was supported by EuroNanoMed3 TEMPEAT and SIRIC Brio Commucan projects. We also thank financial support to MINECO (PID2020-116490GB-I00, Porphyrinoids) and PCIN-2017-042, TEMPEAT), the Comunidad de Madrid (MAD2D-CM) and MICINN through project “Materiales disruptivos bidimensionales (2D)” within the Planes Complementarios (Materiales avanzados). The Nouvelle Aquitaine Region and the FRM are also thanked for financial support. IMDEA Nanociencia acknowledges support from the ‘‘Severo Ochoa’’ Programme for Centres of Excellence in R&D (MINECO, Grant SEV-2016-0686).

Funding Information:
This work was supported by EuroNanoMed3 TEMPEAT and SIRIC Brio Commucan projects. We also thank financial support to MINECO (PID2020‐116490GB‐I00, Porphyrinoids) and PCIN‐2017‐042, TEMPEAT), the Comunidad de Madrid (MAD2D‐CM) and MICINN through project “Materiales disruptivos bidimensionales (2D)” within the Planes Complementarios (Materiales avanzados). The Nouvelle Aquitaine Region and the FRM are also thanked for financial support. IMDEA Nanociencia acknowledges support from the ‘‘Severo Ochoa’’ Programme for Centres of Excellence in R&D (MINECO, Grant SEV‐2016‐0686).

Publisher Copyright:
© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

Funding

This work was supported by EuroNanoMed3 TEMPEAT and SIRIC Brio Commucan projects. We also thank financial support to MINECO (PID2020-116490GB-I00, Porphyrinoids) and PCIN-2017-042, TEMPEAT), the Comunidad de Madrid (MAD2D-CM) and MICINN through project “Materiales disruptivos bidimensionales (2D)” within the Planes Complementarios (Materiales avanzados). The Nouvelle Aquitaine Region and the FRM are also thanked for financial support. IMDEA Nanociencia acknowledges support from the ‘‘Severo Ochoa’’ Programme for Centres of Excellence in R&D (MINECO, Grant SEV-2016-0686). This work was supported by EuroNanoMed3 TEMPEAT and SIRIC Brio Commucan projects. We also thank financial support to MINECO (PID2020‐116490GB‐I00, Porphyrinoids) and PCIN‐2017‐042, TEMPEAT), the Comunidad de Madrid (MAD2D‐CM) and MICINN through project “Materiales disruptivos bidimensionales (2D)” within the Planes Complementarios (Materiales avanzados). The Nouvelle Aquitaine Region and the FRM are also thanked for financial support. IMDEA Nanociencia acknowledges support from the ‘‘Severo Ochoa’’ Programme for Centres of Excellence in R&D (MINECO, Grant SEV‐2016‐0686).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Elastin-Like Peptides
Nanomedicine
Photodynamic Therapy
Self-Assembly
Stimuli-Responsive
Peptides/chemistry
Micelles
Nanoparticles/chemistry
Elastin/chemistry

Access to Document

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Cite this

Le, D. H. T., Ibrahimova, V., van den Wildenberg, S. A. H., Wu, H., Fonseca, A., Torres, T., Garanger, E., Leenders, W. P. J., Brock, R., Lecommandoux, S., & van Hest, J. C. M. (2023). Light-Responsive Elastin-Like Peptide-Based Targeted Nanoparticles for Enhanced Spheroid Penetration. Angewandte Chemie - International Edition, 62(24), Article e202300511. https://doi.org/10.1002/anie.202300511

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title = "Light-Responsive Elastin-Like Peptide-Based Targeted Nanoparticles for Enhanced Spheroid Penetration",

abstract = "We describe here a near infrared light-responsive elastin-like peptide (ELP)-based targeted nanoparticle (NP) that can rapidly switch its size from 120 to 25 nm upon photo-irradiation. Interestingly, the targeting function, which is crucial for effective cargo delivery, is preserved after transformation. The NPs are assembled from (targeted) diblock ELP micelles encapsulating photosensitizer TT1-monoblock ELP conjugates. Methionine residues in this monoblock are photo-oxidized by singlet oxygen generated from TT1, turning the ELPs hydrophilic and thus trigger NP dissociation. Phenylalanine residues from the diblocks then interact with TT1 via π-π stacking, inducing the re-formation of smaller NPs. Due to their small size and targeting function, the NPs penetrate deeper in spheroids and kill cancer cells more efficiently compared to the larger ones. This work could contribute to the design of {\textquotedblleft}smart{\textquotedblright} nanomedicines with deeper penetration capacity for effective anticancer therapies.",

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note = "Funding Information: This work was supported by EuroNanoMed3 TEMPEAT and SIRIC Brio Commucan projects. We also thank financial support to MINECO (PID2020-116490GB-I00, Porphyrinoids) and PCIN-2017-042, TEMPEAT), the Comunidad de Madrid (MAD2D-CM) and MICINN through project {\textquotedblleft}Materiales disruptivos bidimensionales (2D){\textquotedblright} within the Planes Complementarios (Materiales avanzados). The Nouvelle Aquitaine Region and the FRM are also thanked for financial support. IMDEA Nanociencia acknowledges support from the {\textquoteleft}{\textquoteleft}Severo Ochoa{\textquoteright}{\textquoteright} Programme for Centres of Excellence in R\&D (MINECO, Grant SEV-2016-0686). Funding Information: This work was supported by EuroNanoMed3 TEMPEAT and SIRIC Brio Commucan projects. We also thank financial support to MINECO (PID2020‐116490GB‐I00, Porphyrinoids) and PCIN‐2017‐042, TEMPEAT), the Comunidad de Madrid (MAD2D‐CM) and MICINN through project {\textquotedblleft}Materiales disruptivos bidimensionales (2D){\textquotedblright} within the Planes Complementarios (Materiales avanzados). The Nouvelle Aquitaine Region and the FRM are also thanked for financial support. IMDEA Nanociencia acknowledges support from the {\textquoteleft}{\textquoteleft}Severo Ochoa{\textquoteright}{\textquoteright} Programme for Centres of Excellence in R\&D (MINECO, Grant SEV‐2016‐0686). Publisher Copyright: {\textcopyright} 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.",

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month = jun,

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doi = "10.1002/anie.202300511",

language = "English",

volume = "62",

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T1 - Light-Responsive Elastin-Like Peptide-Based Targeted Nanoparticles for Enhanced Spheroid Penetration

AU - Le, Duc H.T.

AU - Ibrahimova, Vusala

AU - van den Wildenberg, Sebastian A.H.

AU - Wu, Hanglong

AU - Fonseca, Alba

AU - Torres, Tomas

AU - Garanger, Elisabeth

AU - Leenders, William P.J.

AU - Brock, Roland

AU - Lecommandoux, Sébastien

AU - van Hest, Jan C.M.

N1 - Funding Information: This work was supported by EuroNanoMed3 TEMPEAT and SIRIC Brio Commucan projects. We also thank financial support to MINECO (PID2020-116490GB-I00, Porphyrinoids) and PCIN-2017-042, TEMPEAT), the Comunidad de Madrid (MAD2D-CM) and MICINN through project “Materiales disruptivos bidimensionales (2D)” within the Planes Complementarios (Materiales avanzados). The Nouvelle Aquitaine Region and the FRM are also thanked for financial support. IMDEA Nanociencia acknowledges support from the ‘‘Severo Ochoa’’ Programme for Centres of Excellence in R&D (MINECO, Grant SEV-2016-0686). Funding Information: This work was supported by EuroNanoMed3 TEMPEAT and SIRIC Brio Commucan projects. We also thank financial support to MINECO (PID2020‐116490GB‐I00, Porphyrinoids) and PCIN‐2017‐042, TEMPEAT), the Comunidad de Madrid (MAD2D‐CM) and MICINN through project “Materiales disruptivos bidimensionales (2D)” within the Planes Complementarios (Materiales avanzados). The Nouvelle Aquitaine Region and the FRM are also thanked for financial support. IMDEA Nanociencia acknowledges support from the ‘‘Severo Ochoa’’ Programme for Centres of Excellence in R&D (MINECO, Grant SEV‐2016‐0686). Publisher Copyright: © 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

PY - 2023/6/12

Y1 - 2023/6/12

N2 - We describe here a near infrared light-responsive elastin-like peptide (ELP)-based targeted nanoparticle (NP) that can rapidly switch its size from 120 to 25 nm upon photo-irradiation. Interestingly, the targeting function, which is crucial for effective cargo delivery, is preserved after transformation. The NPs are assembled from (targeted) diblock ELP micelles encapsulating photosensitizer TT1-monoblock ELP conjugates. Methionine residues in this monoblock are photo-oxidized by singlet oxygen generated from TT1, turning the ELPs hydrophilic and thus trigger NP dissociation. Phenylalanine residues from the diblocks then interact with TT1 via π-π stacking, inducing the re-formation of smaller NPs. Due to their small size and targeting function, the NPs penetrate deeper in spheroids and kill cancer cells more efficiently compared to the larger ones. This work could contribute to the design of “smart” nanomedicines with deeper penetration capacity for effective anticancer therapies.

AB - We describe here a near infrared light-responsive elastin-like peptide (ELP)-based targeted nanoparticle (NP) that can rapidly switch its size from 120 to 25 nm upon photo-irradiation. Interestingly, the targeting function, which is crucial for effective cargo delivery, is preserved after transformation. The NPs are assembled from (targeted) diblock ELP micelles encapsulating photosensitizer TT1-monoblock ELP conjugates. Methionine residues in this monoblock are photo-oxidized by singlet oxygen generated from TT1, turning the ELPs hydrophilic and thus trigger NP dissociation. Phenylalanine residues from the diblocks then interact with TT1 via π-π stacking, inducing the re-formation of smaller NPs. Due to their small size and targeting function, the NPs penetrate deeper in spheroids and kill cancer cells more efficiently compared to the larger ones. This work could contribute to the design of “smart” nanomedicines with deeper penetration capacity for effective anticancer therapies.

KW - Elastin-Like Peptides

KW - Nanomedicine

KW - Photodynamic Therapy

KW - Self-Assembly

KW - Stimuli-Responsive

KW - Peptides/chemistry

KW - Micelles

KW - Nanoparticles/chemistry

KW - Elastin/chemistry

UR - https://www.scopus.com/pages/publications/85158098341

U2 - 10.1002/anie.202300511

DO - 10.1002/anie.202300511

M3 - Article

C2 - 37083071

AN - SCOPUS:85158098341

SN - 1433-7851

VL - 62

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 24

M1 - e202300511

ER -

Light-Responsive Elastin-Like Peptide-Based Targeted Nanoparticles for Enhanced Spheroid Penetration

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

Access to Document

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