Kinetic analysis of horseradish peroxidase"wiring" in redox polyelectrolyte-peroxidase multilayer assemblies

V. Rosca, I.C. Popescu

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)

Abstract

A hydrogen peroxide sensitive interface based on a self-deposited redox polyelectrolyte–peroxidase assembly was used as model system for investigation of the electron transfer between chemically modified horseradish peroxidase and the redox active centers of poly[(vinylpyridine)Os(bpy)2Cl] redox polymer. The assembly was built up by sequential adsorption of the redox polymer and the peroxidase on gold electrodes modified with a self-assembled monolayer of 3-mercapto-1-propane sulfonic acid. Making use of a kinetic model, formulated for steady-state conditions, and of the rotating disc electrode technique it was possible to estimate: (i) the second order rate constant of reaction between the modified peroxidase and hydrogen peroxide (2.74×105 M-1 s-1) and (ii) the apparent turnover number of the oxidized enzyme regeneration by the osmium redox center (2.14 s-1), i.e., two parameters that reflect the efficiency of the electrical communication within the investigated interface.
Original languageEnglish
Pages (from-to)904-911
JournalElectrochemistry Communications
Volume4
Issue number11
DOIs
Publication statusPublished - 2002

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