Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery

Sandra Jimenez-Falcao; Natalia Joga; Alba García-Fernández; Antoni Llopis Lorente; Daniel Torres; Beatriz De Luis; Félix Sancenón; Paloma Martínez-Ruiz; Ramón Martínez-Máñez; Reynaldo Villalonga

doi:10.1039/c9tb00938h

Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery

Sandra Jimenez-Falcao
, Natalia Joga
, Alba García-Fernández
, Antoni Llopis Lorente
, Daniel Torres
, Beatriz De Luis
, Félix Sancenón
, Paloma Martínez-Ruiz
, Ramón Martínez-Máñez
, Reynaldo Villalonga

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

Abstract

We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.

Original language	English
Pages (from-to)	4669-4676
Number of pages	8
Journal	Journal of Materials Chemistry B
Volume	7
Issue number	30
DOIs	https://doi.org/10.1039/c9tb00938h
Publication status	Published - 31 Jul 2019
Externally published	Yes

Funding

Financial support from the Spanish Ministry of Economy and Competitiveness (projects CTQ2014-58989-P, CTQ2015-71936-REDT, CTQ2017-87954-P, MAT2015-64139-C4-1 and RTI2018-100910-B-C41) is gratefully acknowledged. The authors also thank the Generalitat Valencia (Project PROMETEO/2018/024) for support.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1039/c9tb00938h

Cite this

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title = "Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery",

abstract = "We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.",

author = "Sandra Jimenez-Falcao and Natalia Joga and Alba Garc{\'i}a-Fern{\'a}ndez and \{Llopis Lorente\}, Antoni and Daniel Torres and \{De Luis\}, Beatriz and F{\'e}lix Sancen{\'o}n and Paloma Mart{\'i}nez-Ruiz and Ram{\'o}n Mart{\'i}nez-M{\'a}{\~n}ez and Reynaldo Villalonga",

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doi = "10.1039/c9tb00938h",

language = "English",

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T1 - Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery

AU - Jimenez-Falcao, Sandra

AU - Joga, Natalia

AU - García-Fernández, Alba

AU - Llopis Lorente, Antoni

AU - Torres, Daniel

AU - De Luis, Beatriz

AU - Sancenón, Félix

AU - Martínez-Ruiz, Paloma

AU - Martínez-Máñez, Ramón

AU - Villalonga, Reynaldo

PY - 2019/7/31

Y1 - 2019/7/31

N2 - We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.

AB - We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.

UR - https://www.scopus.com/pages/publications/85070100214

U2 - 10.1039/c9tb00938h

DO - 10.1039/c9tb00938h

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JO - Journal of Materials Chemistry B

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ER -

Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery

Abstract

Funding

UN SDGs

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