Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery

Sandra Jimenez-Falcao, Natalia Joga, Alba García-Fernández, Antoni Llopis Lorente, Daniel Torres, Beatriz De Luis, Félix Sancenón, Paloma Martínez-Ruiz, Ramón Martínez-Máñez, Reynaldo Villalonga

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.

Original languageEnglish
Pages (from-to)4669-4676
Number of pages8
JournalJournal of Materials Chemistry B
Volume7
Issue number30
DOIs
Publication statusPublished - 31 Jul 2019
Externally publishedYes

Fingerprint

beta-Fructofuranosidase
Glucose Oxidase
Glucose oxidase
Nanoparticles
Cyclodextrins
Sugar (sucrose)
Silicon Dioxide
Gold
Doxorubicin
Glucose
Sucrose
Hydrolysis
Enzymes
Silica
Cells
Acids
gluconic acid
benzimidazole

Cite this

Jimenez-Falcao, S., Joga, N., García-Fernández, A., Llopis Lorente, A., Torres, D., De Luis, B., ... Villalonga, R. (2019). Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery. Journal of Materials Chemistry B, 7(30), 4669-4676. https://doi.org/10.1039/c9tb00938h
Jimenez-Falcao, Sandra ; Joga, Natalia ; García-Fernández, Alba ; Llopis Lorente, Antoni ; Torres, Daniel ; De Luis, Beatriz ; Sancenón, Félix ; Martínez-Ruiz, Paloma ; Martínez-Máñez, Ramón ; Villalonga, Reynaldo. / Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery. In: Journal of Materials Chemistry B. 2019 ; Vol. 7, No. 30. pp. 4669-4676.
@article{9c7fcb43a9de40b48a17ac09c6b236c9,
title = "Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery",
abstract = "We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.",
author = "Sandra Jimenez-Falcao and Natalia Joga and Alba Garc{\'i}a-Fern{\'a}ndez and {Llopis Lorente}, Antoni and Daniel Torres and {De Luis}, Beatriz and F{\'e}lix Sancen{\'o}n and Paloma Mart{\'i}nez-Ruiz and Ram{\'o}n Mart{\'i}nez-M{\'a}{\~n}ez and Reynaldo Villalonga",
year = "2019",
month = "7",
day = "31",
doi = "10.1039/c9tb00938h",
language = "English",
volume = "7",
pages = "4669--4676",
journal = "Journal of Materials Chemistry B",
issn = "2050-750X",
publisher = "Royal Society of Chemistry",
number = "30",

}

Jimenez-Falcao, S, Joga, N, García-Fernández, A, Llopis Lorente, A, Torres, D, De Luis, B, Sancenón, F, Martínez-Ruiz, P, Martínez-Máñez, R & Villalonga, R 2019, 'Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery', Journal of Materials Chemistry B, vol. 7, no. 30, pp. 4669-4676. https://doi.org/10.1039/c9tb00938h

Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery. / Jimenez-Falcao, Sandra; Joga, Natalia; García-Fernández, Alba; Llopis Lorente, Antoni; Torres, Daniel; De Luis, Beatriz; Sancenón, Félix; Martínez-Ruiz, Paloma; Martínez-Máñez, Ramón; Villalonga, Reynaldo.

In: Journal of Materials Chemistry B, Vol. 7, No. 30, 31.07.2019, p. 4669-4676.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery

AU - Jimenez-Falcao, Sandra

AU - Joga, Natalia

AU - García-Fernández, Alba

AU - Llopis Lorente, Antoni

AU - Torres, Daniel

AU - De Luis, Beatriz

AU - Sancenón, Félix

AU - Martínez-Ruiz, Paloma

AU - Martínez-Máñez, Ramón

AU - Villalonga, Reynaldo

PY - 2019/7/31

Y1 - 2019/7/31

N2 - We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.

AB - We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=85070100214&partnerID=8YFLogxK

U2 - 10.1039/c9tb00938h

DO - 10.1039/c9tb00938h

M3 - Article

C2 - 31364688

AN - SCOPUS:85070100214

VL - 7

SP - 4669

EP - 4676

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-750X

IS - 30

ER -

Jimenez-Falcao S, Joga N, García-Fernández A, Llopis Lorente A, Torres D, De Luis B et al. Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery. Journal of Materials Chemistry B. 2019 Jul 31;7(30):4669-4676. https://doi.org/10.1039/c9tb00938h