Intrinsic isotachophoretic preconcentration in capillary gel electrophoresis of DNA restriction fragments

M.J. Schans, van der; J.L. Beckers; M.C. Molling; F.M. Everaerts

doi:10.1016/0021-9673(95)00534-6

Intrinsic isotachophoretic preconcentration in capillary gel electrophoresis of DNA restriction fragments

M.J. Schans, van der, J.L. Beckers, M.C. Molling, F.M. Everaerts

Chemical Engineering and Chemistry

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Abstract

Reproducible migration times are required for accurate base-pair assignment of polymerase chain reaction (PCR) products in capillary gel electrophoresis (CGE). However, migration times are influenced by injection plug length and ionic strength of the sample. In this paper we introduce a new isotachophoresis (ITP)-CGE system where the transition from ITP to CGE is achieved by the mobility shift of DNA from free solution (ITP) to sieving gel buffer (CGE). With intrinsic isotachophoretic preconcentration in capillary gel electrophoresis (IICGE) large volume injections (up to 700 nl) are possible with accurate migration times in CGE independent of injection plug length or sample ionic strength.

Original language	English
Pages (from-to)	139-147
Number of pages	10
Journal	Journal of Chromatography, A
Volume	717
Issue number	1-2
DOIs	https://doi.org/10.1016/0021-9673(95)00534-6
Publication status	Published - 1995

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title = "Intrinsic isotachophoretic preconcentration in capillary gel electrophoresis of DNA restriction fragments",

abstract = "Reproducible migration times are required for accurate base-pair assignment of polymerase chain reaction (PCR) products in capillary gel electrophoresis (CGE). However, migration times are influenced by injection plug length and ionic strength of the sample. In this paper we introduce a new isotachophoresis (ITP)-CGE system where the transition from ITP to CGE is achieved by the mobility shift of DNA from free solution (ITP) to sieving gel buffer (CGE). With intrinsic isotachophoretic preconcentration in capillary gel electrophoresis (IICGE) large volume injections (up to 700 nl) are possible with accurate migration times in CGE independent of injection plug length or sample ionic strength.",

author = "{Schans, van der}, M.J. and J.L. Beckers and M.C. Molling and F.M. Everaerts",

year = "1995",

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T1 - Intrinsic isotachophoretic preconcentration in capillary gel electrophoresis of DNA restriction fragments

AU - Schans, van der, M.J.

AU - Beckers, J.L.

AU - Molling, M.C.

AU - Everaerts, F.M.

PY - 1995

Y1 - 1995

N2 - Reproducible migration times are required for accurate base-pair assignment of polymerase chain reaction (PCR) products in capillary gel electrophoresis (CGE). However, migration times are influenced by injection plug length and ionic strength of the sample. In this paper we introduce a new isotachophoresis (ITP)-CGE system where the transition from ITP to CGE is achieved by the mobility shift of DNA from free solution (ITP) to sieving gel buffer (CGE). With intrinsic isotachophoretic preconcentration in capillary gel electrophoresis (IICGE) large volume injections (up to 700 nl) are possible with accurate migration times in CGE independent of injection plug length or sample ionic strength.

AB - Reproducible migration times are required for accurate base-pair assignment of polymerase chain reaction (PCR) products in capillary gel electrophoresis (CGE). However, migration times are influenced by injection plug length and ionic strength of the sample. In this paper we introduce a new isotachophoresis (ITP)-CGE system where the transition from ITP to CGE is achieved by the mobility shift of DNA from free solution (ITP) to sieving gel buffer (CGE). With intrinsic isotachophoretic preconcentration in capillary gel electrophoresis (IICGE) large volume injections (up to 700 nl) are possible with accurate migration times in CGE independent of injection plug length or sample ionic strength.

U2 - 10.1016/0021-9673(95)00534-6

DO - 10.1016/0021-9673(95)00534-6

M3 - Article

SN - 0021-9673

VL - 717

SP - 139

EP - 147

JO - Journal of Chromatography, A

JF - Journal of Chromatography, A

IS - 1-2

ER -

Intrinsic isotachophoretic preconcentration in capillary gel electrophoresis of DNA restriction fragments

Abstract

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