Intermediate filament protein partnership in astrocytes

C. Eliasson, C. Sahlgren, C.-H. Berthold, J. Stakeberg, J.E. Celis, C. Betsholtz, M. Pekny

    Research output: Contribution to journalArticleAcademicpeer-review

    278 Citations (Scopus)

    Abstract

    Intermediate filaments are general constituents of the cytoskeleton. The function of these structures and the requirement for different types of intermediate filament proteins by individual cells are only partly understood. Here we have addressed the role of specific intermediate filament protein partnerships in the formation of intermediate filaments in astrocytes. Astrocytes may express three types of intermediate filament proteins: glial fibrillary acidic protein (GFAP), vimentin, and nestin. We used mice with targeted mutations in the GFAP or vimentin genes, or both, to study the impact of loss of either or both of these proteins on intermediate filament formation in cultured astrocytes and in normal or reactive astrocytes in vivo. We report that nestin cannot form intermediate filaments on its own, that vimentin may form intermediate filaments with either nestin or GFAP as obligatory partners, and that GFAP is the only intermediate filament protein of the three that may form filaments on its own. However, such filaments show abnormal organization. Aberrant intermediate filament formation is linked to diseases affecting epithelial, neuronal, and muscle cells. Here we present models by which the normal and pathogenic functions of intermediate filaments may be elucidated in astrocytes.
    Original languageEnglish
    Pages (from-to)23996-24006
    Number of pages11
    JournalJournal of Biological Chemistry
    Volume274
    Issue number34
    DOIs
    Publication statusPublished - 1999

    Fingerprint

    Intermediate Filament Proteins
    Intermediate Filaments
    Astrocytes
    Glial Fibrillary Acidic Protein
    Nestin
    Vimentin
    Muscle
    Cytoskeleton
    Muscle Cells
    Genes
    Cells
    Proteins
    Epithelial Cells
    Mutation

    Cite this

    Eliasson, C., Sahlgren, C., Berthold, C-H., Stakeberg, J., Celis, J. E., Betsholtz, C., & Pekny, M. (1999). Intermediate filament protein partnership in astrocytes. Journal of Biological Chemistry, 274(34), 23996-24006. https://doi.org/10.1074/jbc.274.34.23996
    Eliasson, C. ; Sahlgren, C. ; Berthold, C.-H. ; Stakeberg, J. ; Celis, J.E. ; Betsholtz, C. ; Pekny, M. / Intermediate filament protein partnership in astrocytes. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 34. pp. 23996-24006.
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    Eliasson, C, Sahlgren, C, Berthold, C-H, Stakeberg, J, Celis, JE, Betsholtz, C & Pekny, M 1999, 'Intermediate filament protein partnership in astrocytes', Journal of Biological Chemistry, vol. 274, no. 34, pp. 23996-24006. https://doi.org/10.1074/jbc.274.34.23996

    Intermediate filament protein partnership in astrocytes. / Eliasson, C.; Sahlgren, C.; Berthold, C.-H.; Stakeberg, J.; Celis, J.E.; Betsholtz, C.; Pekny, M.

    In: Journal of Biological Chemistry, Vol. 274, No. 34, 1999, p. 23996-24006.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - Intermediate filament protein partnership in astrocytes

    AU - Eliasson, C.

    AU - Sahlgren, C.

    AU - Berthold, C.-H.

    AU - Stakeberg, J.

    AU - Celis, J.E.

    AU - Betsholtz, C.

    AU - Pekny, M.

    PY - 1999

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    N2 - Intermediate filaments are general constituents of the cytoskeleton. The function of these structures and the requirement for different types of intermediate filament proteins by individual cells are only partly understood. Here we have addressed the role of specific intermediate filament protein partnerships in the formation of intermediate filaments in astrocytes. Astrocytes may express three types of intermediate filament proteins: glial fibrillary acidic protein (GFAP), vimentin, and nestin. We used mice with targeted mutations in the GFAP or vimentin genes, or both, to study the impact of loss of either or both of these proteins on intermediate filament formation in cultured astrocytes and in normal or reactive astrocytes in vivo. We report that nestin cannot form intermediate filaments on its own, that vimentin may form intermediate filaments with either nestin or GFAP as obligatory partners, and that GFAP is the only intermediate filament protein of the three that may form filaments on its own. However, such filaments show abnormal organization. Aberrant intermediate filament formation is linked to diseases affecting epithelial, neuronal, and muscle cells. Here we present models by which the normal and pathogenic functions of intermediate filaments may be elucidated in astrocytes.

    AB - Intermediate filaments are general constituents of the cytoskeleton. The function of these structures and the requirement for different types of intermediate filament proteins by individual cells are only partly understood. Here we have addressed the role of specific intermediate filament protein partnerships in the formation of intermediate filaments in astrocytes. Astrocytes may express three types of intermediate filament proteins: glial fibrillary acidic protein (GFAP), vimentin, and nestin. We used mice with targeted mutations in the GFAP or vimentin genes, or both, to study the impact of loss of either or both of these proteins on intermediate filament formation in cultured astrocytes and in normal or reactive astrocytes in vivo. We report that nestin cannot form intermediate filaments on its own, that vimentin may form intermediate filaments with either nestin or GFAP as obligatory partners, and that GFAP is the only intermediate filament protein of the three that may form filaments on its own. However, such filaments show abnormal organization. Aberrant intermediate filament formation is linked to diseases affecting epithelial, neuronal, and muscle cells. Here we present models by which the normal and pathogenic functions of intermediate filaments may be elucidated in astrocytes.

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    Eliasson C, Sahlgren C, Berthold C-H, Stakeberg J, Celis JE, Betsholtz C et al. Intermediate filament protein partnership in astrocytes. Journal of Biological Chemistry. 1999;274(34):23996-24006. https://doi.org/10.1074/jbc.274.34.23996