Extracellular matrix (ECM), as a bio-chemical and -physical support for cells, is of great importance in cell migration studies. 3D migration studies, compared to 2D cultures, have proven to best represent the in vivo conditions. Hydrogels are usually used in in vitro studies as the 3D ECM. However, the relevance of the architecture and controllability of gels are debatable. Self standing fibrous scaffolds, which more closely mimic the in vivo condition, can be fabricated (by electro-spinning) with different fiber sizes and architecture and from different materials. In addition, microfluidic chips can intrinsically control the biochemical content of the cell micro-environment which is also important for the cell migration. In this project, we have developed a new micro-fabrication method to integrate fibrous scaffolds inside a microfluidic device to study cell migration on a chip.
|Publication status||Published - Nov 2015|