Integrating extracellular matrix-mimicking layers in microfluidic devices: More control over the tumor microenvironment

Hossein Eslami Amirabadi; Sheen SahebAli; Jean Philippe Frimat; Regina Luttge; Jaap M.J. den Toonder

Integrating extracellular matrix-mimicking layers in microfluidic devices: More control over the tumor microenvironment

Hossein Eslami Amirabadi, Sheen SahebAli, Jean Philippe Frimat, Regina Luttge, Jaap M.J. den Toonder

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

A challenge in studying cancer invasion is to understand the interaction between cancer cells and the extracellular matrix (ECM). Although the ECM influences cancer invasion, it is still unclear how its properties contribute to the process. Here, we present a new microfabrication method to integrate ECM-mimicking matrix layers between two microchannels. Using this chip, we have observed that the invasion distance of MDA-MB-231 breast cancer cells into 3D Polycaprolactone matrices and the general cell morphology are not affected by the ECM fiber diameter. The cells however produce longer and more protrusions in the matrix with smaller fiber size.

Original language	English
Title of host publication	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Publisher	Chemical and Biological Microsystems Society
Pages	1157-1158
Number of pages	2
ISBN (Electronic)	9780692941836
Publication status	Published - 2017
Event	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah International Trade & Convention Center 1 International Drive Savannah, Georgia 31402 USA, Savannah, United States Duration: 22 Oct 2017 → 26 Oct 2017 Conference number: 21 http://www.microtasconferences.org/microtas2017/

Conference

Conference	21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Abbreviated title	MicroTAS 2017
Country/Territory	United States
City	Savannah
Period	22/10/17 → 26/10/17
Internet address	http://www.microtasconferences.org/microtas2017/

Keywords

3D Matrix Layers
Cancer Cell Invasion
ECM Architecture
Microfabrication
Microfluidics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Amirabadi, H. E., SahebAli, S., Frimat, J. P., Luttge, R., & den Toonder, J. M. J. (2017). Integrating extracellular matrix-mimicking layers in microfluidic devices: More control over the tumor microenvironment. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 1157-1158). Chemical and Biological Microsystems Society.

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title = "Integrating extracellular matrix-mimicking layers in microfluidic devices: More control over the tumor microenvironment",

abstract = "A challenge in studying cancer invasion is to understand the interaction between cancer cells and the extracellular matrix (ECM). Although the ECM influences cancer invasion, it is still unclear how its properties contribute to the process. Here, we present a new microfabrication method to integrate ECM-mimicking matrix layers between two microchannels. Using this chip, we have observed that the invasion distance of MDA-MB-231 breast cancer cells into 3D Polycaprolactone matrices and the general cell morphology are not affected by the ECM fiber diameter. The cells however produce longer and more protrusions in the matrix with smaller fiber size.",

keywords = "3D Matrix Layers, Cancer Cell Invasion, ECM Architecture, Microfabrication, Microfluidics",

author = "Amirabadi, {Hossein Eslami} and Sheen SahebAli and Frimat, {Jean Philippe} and Regina Luttge and {den Toonder}, {Jaap M.J.}",

year = "2017",

language = "English",

pages = "1157--1158",

booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",

publisher = "Chemical and Biological Microsystems Society",

note = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, MicroTAS 2017 ; Conference date: 22-10-2017 Through 26-10-2017",

url = "http://www.microtasconferences.org/microtas2017/",

}

Amirabadi, HE, SahebAli, S, Frimat, JP, Luttge, R & den Toonder, JMJ 2017, Integrating extracellular matrix-mimicking layers in microfluidic devices: More control over the tumor microenvironment. in 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, pp. 1157-1158, 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017, Savannah, United States, 22/10/17.

Integrating extracellular matrix-mimicking layers in microfluidic devices: More control over the tumor microenvironment. / Amirabadi, Hossein Eslami; SahebAli, Sheen; Frimat, Jean Philippe et al.
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017. Chemical and Biological Microsystems Society, 2017. p. 1157-1158.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Integrating extracellular matrix-mimicking layers in microfluidic devices

T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

AU - Amirabadi, Hossein Eslami

AU - SahebAli, Sheen

AU - Frimat, Jean Philippe

AU - Luttge, Regina

AU - den Toonder, Jaap M.J.

N1 - Conference code: 21

PY - 2017

Y1 - 2017

N2 - A challenge in studying cancer invasion is to understand the interaction between cancer cells and the extracellular matrix (ECM). Although the ECM influences cancer invasion, it is still unclear how its properties contribute to the process. Here, we present a new microfabrication method to integrate ECM-mimicking matrix layers between two microchannels. Using this chip, we have observed that the invasion distance of MDA-MB-231 breast cancer cells into 3D Polycaprolactone matrices and the general cell morphology are not affected by the ECM fiber diameter. The cells however produce longer and more protrusions in the matrix with smaller fiber size.

AB - A challenge in studying cancer invasion is to understand the interaction between cancer cells and the extracellular matrix (ECM). Although the ECM influences cancer invasion, it is still unclear how its properties contribute to the process. Here, we present a new microfabrication method to integrate ECM-mimicking matrix layers between two microchannels. Using this chip, we have observed that the invasion distance of MDA-MB-231 breast cancer cells into 3D Polycaprolactone matrices and the general cell morphology are not affected by the ECM fiber diameter. The cells however produce longer and more protrusions in the matrix with smaller fiber size.

KW - 3D Matrix Layers

KW - Cancer Cell Invasion

KW - ECM Architecture

KW - Microfabrication

KW - Microfluidics

UR - http://www.scopus.com/inward/record.url?scp=85079660028&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85079660028

SP - 1157

EP - 1158

BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

PB - Chemical and Biological Microsystems Society

Y2 - 22 October 2017 through 26 October 2017

ER -

Integrating extracellular matrix-mimicking layers in microfluidic devices: More control over the tumor microenvironment

Abstract

Conference

Keywords

UN SDGs

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