Injectable porous microspheres form highly porous scaffolds after injection

O. Qutachi, J.R. Vetsch, D. Gill, H. Cox, D.J. Scurr, S. Hofmann, R. Müller, R.A. Quirk, K.M. Shakesheff, C.V. Rahman

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Abstract

Injectable scaffolds are of interest in the field of regenerative medicine because of their minimally invasive mode of delivery. For tissue repair applications, it is essential that such scaffolds have the mechanical properties, porosity and pore diameter to support the formation of new tissue. In the current study, porous poly(dl-lactic acid-co-glycolic acid) (PLGA) microspheres were fabricated with an average size of 84 ± 24 µm for use as injectable cell carriers. Treatment with ethanolic sodium hydroxide for 2 min was observed to increase surface porosity without causing the microsphere structure to disintegrate. This surface treatment also enabled the microspheres to fuse together at 37 °C to form scaffold structures. The average compressive strength of the scaffolds after 24 h at 37 °C was 0.9 ± 0.1 MPa, and the average Young’s modulus was 9.4 ± 1.2 MPa. Scaffold porosity levels were 81.6% on average, with a mean pore diameter of 54 ± 38 µm. This study demonstrates a method for fabricating porous PLGA microspheres that form solid porous scaffolds at body temperature, creating an injectable system capable of supporting NIH-3T3 cell attachment and proliferation in vitro.
Original languageEnglish
Pages (from-to)5090-5098
Number of pages8
JournalActa Biomaterialia
Volume10
Issue number12
DOIs
Publication statusPublished - 2014

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Forms (concrete)
Microspheres
Scaffolds
Porosity
Injections
glycolic acid
Compressive Strength
NIH 3T3 Cells
Sodium Hydroxide
Regenerative Medicine
Elastic Modulus
Body Temperature
Tissue
Cell Proliferation
Electric fuses
Lactic acid
Compressive strength
Surface treatment
Lactic Acid
Repair

Cite this

Qutachi, O., Vetsch, J. R., Gill, D., Cox, H., Scurr, D. J., Hofmann, S., ... Rahman, C. V. (2014). Injectable porous microspheres form highly porous scaffolds after injection. Acta Biomaterialia, 10(12), 5090-5098. https://doi.org/10.1016/j.actbio.2014.08.015
Qutachi, O. ; Vetsch, J.R. ; Gill, D. ; Cox, H. ; Scurr, D.J. ; Hofmann, S. ; Müller, R. ; Quirk, R.A. ; Shakesheff, K.M. ; Rahman, C.V. / Injectable porous microspheres form highly porous scaffolds after injection. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 12. pp. 5090-5098.
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Qutachi, O, Vetsch, JR, Gill, D, Cox, H, Scurr, DJ, Hofmann, S, Müller, R, Quirk, RA, Shakesheff, KM & Rahman, CV 2014, 'Injectable porous microspheres form highly porous scaffolds after injection', Acta Biomaterialia, vol. 10, no. 12, pp. 5090-5098. https://doi.org/10.1016/j.actbio.2014.08.015

Injectable porous microspheres form highly porous scaffolds after injection. / Qutachi, O.; Vetsch, J.R.; Gill, D.; Cox, H.; Scurr, D.J.; Hofmann, S.; Müller, R.; Quirk, R.A.; Shakesheff, K.M.; Rahman, C.V.

In: Acta Biomaterialia, Vol. 10, No. 12, 2014, p. 5090-5098.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Qutachi, O.

AU - Vetsch, J.R.

AU - Gill, D.

AU - Cox, H.

AU - Scurr, D.J.

AU - Hofmann, S.

AU - Müller, R.

AU - Quirk, R.A.

AU - Shakesheff, K.M.

AU - Rahman, C.V.

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AB - Injectable scaffolds are of interest in the field of regenerative medicine because of their minimally invasive mode of delivery. For tissue repair applications, it is essential that such scaffolds have the mechanical properties, porosity and pore diameter to support the formation of new tissue. In the current study, porous poly(dl-lactic acid-co-glycolic acid) (PLGA) microspheres were fabricated with an average size of 84 ± 24 µm for use as injectable cell carriers. Treatment with ethanolic sodium hydroxide for 2 min was observed to increase surface porosity without causing the microsphere structure to disintegrate. This surface treatment also enabled the microspheres to fuse together at 37 °C to form scaffold structures. The average compressive strength of the scaffolds after 24 h at 37 °C was 0.9 ± 0.1 MPa, and the average Young’s modulus was 9.4 ± 1.2 MPa. Scaffold porosity levels were 81.6% on average, with a mean pore diameter of 54 ± 38 µm. This study demonstrates a method for fabricating porous PLGA microspheres that form solid porous scaffolds at body temperature, creating an injectable system capable of supporting NIH-3T3 cell attachment and proliferation in vitro.

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VL - 10

SP - 5090

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JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

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