Influence of batch-to-batch reproducibility of Luna C18(2) packing material, nature of column wall material, and column diameter on the liquid chromatographic analysis of basic analytes

R.J.M. Vervoort, E. Ruyter, A.J.J. Debets, H.A. Claessens, C.A.M.G. Cramers, G.J. Jong, de

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2 Citations (Scopus)

Abstract

This paper discusses aspects arising on transferring liq. chromatog. (LC) methods developed on conventional size columns to micro LC, i.e. the influence of batch-to-batch reproducibility of packing material, the nature of the column wall material, and the column inner diam. It was shown that the nature of the column hardware as well as batches of Luna C18(2) packing material did not influence the retention of basic compds. The batches of packing material, however, significantly influenced the peak shape for some compds. The nature of the mobile phase buffer also showed an effect on peak shape. The column wall material as well as the column inner diam. did not reveal effects on the peak shape. Different packing densities were found for micro and conventional LC columns, i.e. the micro LC columns were less densely packed than the conventional LC columns. The study demonstrated that for the anal. of basic analytes similar sepns. are obtained using micro and conventional LC columns provided that the columns are packed from the same batch of packing material. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)167-172
JournalJournal of Separation Science
Volume24
Issue number3
DOIs
Publication statusPublished - 2001

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