TY - JOUR
T1 - Inflammatory response and bone healing capacity of two porous calcium phosphate ceramics in critical size cortical bone defects
AU - Chatterjea, Anindita
AU - van der Stok, Johan
AU - Danoux, Charlène B.
AU - Yuan, Huipin
AU - Habibovic, Pamela
AU - Van Blitterswijk, Clemens A.
AU - Weinans, Harrie
AU - de Boer, Jan
PY - 2014/5
Y1 - 2014/5
N2 - In the present study, two open porous calcium phosphate ceramics, β-tricalcium phosphate (β-TCP), and hydroxyapatite (HA) were compared in a critical-sized femoral defect in rats. Previous comparisons of these two ceramics showed significantly greater osteoinductive potential of β-TCP upon intramuscular implantation and a better performance in a spinal fusion model in dogs. Results of the current study also showed significantly more bone formation in defects grafted with β-TCP compared to HA; however, both the ceramics were not capable of increasing bone formation to such extend that it bridges the defect. Furthermore, a more pronounced degradation of β-TCP was observed as compared to HA. Progression of inflammation and initiation of new bone formation were assessed for both materials at multiple time points by histological and fluorochrome-based analyses. Until 12 days postimplantation, a strong inflammatory response in absence of new bone formation was observed in both ceramics, without obvious differences between the two materials. Four weeks postimplantation, signs of new bone formation were found in both β-TCP and HA. At 6 weeks, inflammation had subsided in both ceramics while bone deposition continued. In conclusion, the two ceramics differed in the amount of bone formed after 8 weeks of implantation, whereas no differences were found in the duration of the inflammatory phase after implantation or initiation of new bone formation.
AB - In the present study, two open porous calcium phosphate ceramics, β-tricalcium phosphate (β-TCP), and hydroxyapatite (HA) were compared in a critical-sized femoral defect in rats. Previous comparisons of these two ceramics showed significantly greater osteoinductive potential of β-TCP upon intramuscular implantation and a better performance in a spinal fusion model in dogs. Results of the current study also showed significantly more bone formation in defects grafted with β-TCP compared to HA; however, both the ceramics were not capable of increasing bone formation to such extend that it bridges the defect. Furthermore, a more pronounced degradation of β-TCP was observed as compared to HA. Progression of inflammation and initiation of new bone formation were assessed for both materials at multiple time points by histological and fluorochrome-based analyses. Until 12 days postimplantation, a strong inflammatory response in absence of new bone formation was observed in both ceramics, without obvious differences between the two materials. Four weeks postimplantation, signs of new bone formation were found in both β-TCP and HA. At 6 weeks, inflammation had subsided in both ceramics while bone deposition continued. In conclusion, the two ceramics differed in the amount of bone formed after 8 weeks of implantation, whereas no differences were found in the duration of the inflammatory phase after implantation or initiation of new bone formation.
KW - hydroxyapatite
KW - inflammatory response
KW - osteoinductive potential
KW - rat femoral defect
KW - tricalcium phosphate
UR - http://www.scopus.com/inward/record.url?scp=84897112293&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.34815
DO - 10.1002/jbm.a.34815
M3 - Article
C2 - 23733500
AN - SCOPUS:84897112293
SN - 1549-3296
VL - 102
SP - 1399
EP - 1407
JO - Journal of Biomedical Materials Research, Part A
JF - Journal of Biomedical Materials Research, Part A
IS - 5
ER -