In vivo proton T1 relaxation times of mouse myocardial metabolites at 9.4 T

A.J. Bakermans, D. Abdurrachim, T.R. Geraedts, S.M. Houten, K. Nicolay, J.J. Prompers

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    7 Citations (Scopus)

    Abstract

    Purpose Proton magnetic resonance spectroscopy (1H-MRS) for quantitative in vivo assessment of mouse myocardial metabolism requires accurate acquisition timing to minimize motion artifacts and corrections for T1-dependent partial saturation effects. In this study, mouse myocardial water and metabolite T1 relaxation time constants were quantified. Methods Cardiac-triggered and respiratory-gated PRESS-localized 1H-MRS was employed at 9.4 T to acquire signal from a 4-μL voxel in the septum of healthy mice (n=10) while maintaining a steady state of magnetization using dummy scans during respiratory gates. Signal stability was assessed via standard deviations (SD) of zero-order phases and amplitudes of water spectra. Saturation-recovery experiments were performed to determine T1 values. Results Phase SD did not vary for different repetition times (TR), and was 13.1°±4.5°. Maximal amplitude SD was 14.2%±5.1% at TR=500 ms. Myocardial T1 values (mean±SD) were quantified for water (1.71±0.25 s), taurine (2.18±0.62 s), trimethylamine from choline-containing compounds and carnitine (1.67±0.25 s), creatine-methyl (1.34±0.19 s), triglyceride-methylene (0.60±0.15 s), and triglyceride-methyl (0.90±0.17 s) protons. Conclusion This work provides in vivo quantifications of proton T1 values for mouse myocardial water and metabolites at 9.4 T. Magn Reson Med 73:2069-2074, 2015.

    Original languageEnglish
    Pages (from-to)2069-2074
    Number of pages6
    JournalMagnetic Resonance in Medicine
    Volume73
    Issue number6
    DOIs
    Publication statusPublished - 1 Jun 2015

    Keywords

    • <sup>1</sup>H-MRS
    • metabolite T<inf>1</inf> relaxation
    • mouse heart
    • myocardial metabolism
    • PRESS
    • saturation-recovery

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