In vivo postprandial lipid partitioning in liver and muscle of diabetic rats is disturbed

J.J. Prompers, R.A.M. Jonkers, L.J.C. Loon, van, K. Nicolay

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    Abstract

    Objective: To study in vivo lipid partitioning in insulin-resistant liver and muscle of diabetic rats using magnetic resonance spectroscopy (MRS). Methods: Four groups of n=6 male Zucker diabetic fatty rats were used for this study: obese, pre-diabetic fa/fa rats and lean, non-diabetic fa/+ littermates at the age of 6 weeks, and obese, diabetic fa/fa rats and lean, non-diabetic fa/+ littermates at the age of 12 weeks. 1H-[13C] MRS measurements were performed in liver and tibialis anterior muscle at baseline and 4, 24 and 48 h after oral administration of 1.5 g [U-13C] Algal lipid mixture per kg body weight. Results: At baseline, total lipid content was higher in fa/fa rats compared with fa/+ rats in both liver and muscle, and at both ages. Both in pre-diabetic and in diabetic fa/fa rats, hepatic lipid uptake was increased compared with non-diabetic fa/+ rats. Likewise, in muscle of diabetic fa/fa rats, lipid uptake was higher than in muscle of fa/+ rats. In contrast, lipid uptake in muscle of younger, pre-diabetic fa/fa rats was lower than in controls. Conclusion: In the pre-diabetic state, muscle appeared to be protected from massive lipid uptake, whereas lipid uptake in the liver was largely increased. In contrast, after developing full-blown diabetes, lipid uptake was highly elevated in both liver and muscle. This research was funded by a VIDI grant from the Netherlands Organisation for Scientific Research (NWO).
    Original languageEnglish
    Title of host publicationExperimental Biology 2012 : San Diego, April 21-25, 2012
    Pages1014.10
    Publication statusPublished - 2012
    EventExperimental Biology Meeting 2012, April 21-25, 2012, San Diego, CA, USA - San Diego, CA, United States
    Duration: 21 Apr 201225 Apr 2012

    Publication series

    NameFASEB Journal : The Journal of the Federation of American Societies for Experimental Biology
    Volume26
    ISSN (Print)0892-6638

    Conference

    ConferenceExperimental Biology Meeting 2012, April 21-25, 2012, San Diego, CA, USA
    Country/TerritoryUnited States
    CitySan Diego, CA
    Period21/04/1225/04/12

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