Objective: To study in vivo lipid partitioning in insulin-resistant liver and muscle of diabetic rats using magnetic resonance spectroscopy (MRS).
Methods: Four groups of n=6 male Zucker diabetic fatty rats were used for this study: obese, pre-diabetic fa/fa rats and lean, non-diabetic fa/+ littermates at the age of 6 weeks, and obese, diabetic fa/fa rats and lean, non-diabetic fa/+ littermates at the age of 12 weeks. 1H-[13C] MRS measurements were performed in liver and tibialis anterior muscle at baseline and 4, 24 and 48 h after oral administration of 1.5 g [U-13C] Algal lipid mixture per kg body weight.
Results: At baseline, total lipid content was higher in fa/fa rats compared with fa/+ rats in both liver and muscle, and at both ages. Both in pre-diabetic and in diabetic fa/fa rats, hepatic lipid uptake was increased compared with non-diabetic fa/+ rats. Likewise, in muscle of diabetic fa/fa rats, lipid uptake was higher than in muscle of fa/+ rats. In contrast, lipid uptake in muscle of younger, pre-diabetic fa/fa rats was lower than in controls.
Conclusion: In the pre-diabetic state, muscle appeared to be protected from massive lipid uptake, whereas lipid uptake in the liver was largely increased. In contrast, after developing full-blown diabetes, lipid uptake was highly elevated in both liver and muscle. This research was funded by a VIDI grant from the Netherlands Organisation for Scientific Research (NWO).
|Name||FASEB Journal : The Journal of the Federation of American Societies for Experimental Biology|
|Conference||Experimental Biology Meeting 2012, April 21-25, 2012, San Diego, CA, USA|
|City||San Diego, CA|
|Period||21/04/12 → 25/04/12|