In vivo magnetic resonance spectroscopy of lipid handling in steatotic rat liver

S.M.M. Janssens, R.A.M. Jonkers, N.A.W. Riel, van, K. Nicolay, J.J. Prompers

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Objective: Examine lipid handling in liver of rats fed with different high-fat diets using 1H-[13C] magnetic resonance spectroscopy (MRS) together with oral administration of 13C labeled lipids. Methods: 6 male Wistar rats (11 weeks old; 348 ± 8g) were divided into three diet groups: low-fat (10% fat, CON), high-fat lard (45% fat, HFL), and high-fat palm oil (45%, HFP). After 10 weeks of diet, MRS experiments were performed at baseline, and 4 and 24 h after oral administration of 1.5 g [U-13C] Algal lipid mixture per kg body weight. Results: At 4 h after administration of the 13C labeled lipids, 13C enrichment of intracellular liver lipids was similarly increased in all three groups compared to baseline (CON: 0.031 ± 0.017 %; HFL: 0.045 ± 0.022 %; HFP: 0.033 ± 0.013 %), demonstrating that lipid uptake was not affected by the diet regimen. At 24 h, on the other hand, 13C enrichment of liver lipids decreased in CON, whereas in both high-fat diet groups the 13C enrichment did not change compared to 4 h, indicating a lower turnover of the stored liver lipids. Conclusion: High-fat diet feeding did not alter liver lipid uptake in rats, but resulted in a decreased turnover of the lipids stored in the liver. This research was funded by the Netherlands Consortium for Systems Biology (NCSB) which is part of the Netherlands Genomics Initiative/Netherlands Organisation for Scientific Research.
LanguageEnglish
Title of host publicationExperimental Biology 2012 : San Diego, April 21-25, 2012
Pages242.7-
StatePublished - 2012
Eventconference; Experimental Biology MeetingLocation:San Diego, CADate:APR 21-25, 2012 -
Duration: 1 Jan 2012 → …

Publication series

NameFASEB Journal : The Journal of the Federation of American Societies for Experimental Biology
Volume26
ISSN (Print)0892-6638

Conference

Conferenceconference; Experimental Biology MeetingLocation:San Diego, CADate:APR 21-25, 2012
Period1/01/12 → …
OtherExperimental Biology MeetingLocation:San Diego, CADate:APR 21-25, 2012

Fingerprint

Magnetic Resonance Spectroscopy
Lipids
Liver
High Fat Diet
Fats
Netherlands
Oral Administration
Diet
Fat-Restricted Diet
Systems Biology
Genomics
Research
Wistar Rats
Body Weight

Cite this

Janssens, S. M. M., Jonkers, R. A. M., Riel, van, N. A. W., Nicolay, K., & Prompers, J. J. (2012). In vivo magnetic resonance spectroscopy of lipid handling in steatotic rat liver. In Experimental Biology 2012 : San Diego, April 21-25, 2012 (pp. 242.7-). (FASEB Journal : The Journal of the Federation of American Societies for Experimental Biology; Vol. 26).
Janssens, S.M.M. ; Jonkers, R.A.M. ; Riel, van, N.A.W. ; Nicolay, K. ; Prompers, J.J./ In vivo magnetic resonance spectroscopy of lipid handling in steatotic rat liver. Experimental Biology 2012 : San Diego, April 21-25, 2012. 2012. pp. 242.7- (FASEB Journal : The Journal of the Federation of American Societies for Experimental Biology).
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title = "In vivo magnetic resonance spectroscopy of lipid handling in steatotic rat liver",
abstract = "Objective: Examine lipid handling in liver of rats fed with different high-fat diets using 1H-[13C] magnetic resonance spectroscopy (MRS) together with oral administration of 13C labeled lipids. Methods: 6 male Wistar rats (11 weeks old; 348 ± 8g) were divided into three diet groups: low-fat (10{\%} fat, CON), high-fat lard (45{\%} fat, HFL), and high-fat palm oil (45{\%}, HFP). After 10 weeks of diet, MRS experiments were performed at baseline, and 4 and 24 h after oral administration of 1.5 g [U-13C] Algal lipid mixture per kg body weight. Results: At 4 h after administration of the 13C labeled lipids, 13C enrichment of intracellular liver lipids was similarly increased in all three groups compared to baseline (CON: 0.031 ± 0.017 {\%}; HFL: 0.045 ± 0.022 {\%}; HFP: 0.033 ± 0.013 {\%}), demonstrating that lipid uptake was not affected by the diet regimen. At 24 h, on the other hand, 13C enrichment of liver lipids decreased in CON, whereas in both high-fat diet groups the 13C enrichment did not change compared to 4 h, indicating a lower turnover of the stored liver lipids. Conclusion: High-fat diet feeding did not alter liver lipid uptake in rats, but resulted in a decreased turnover of the lipids stored in the liver. This research was funded by the Netherlands Consortium for Systems Biology (NCSB) which is part of the Netherlands Genomics Initiative/Netherlands Organisation for Scientific Research.",
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Janssens, SMM, Jonkers, RAM, Riel, van, NAW, Nicolay, K & Prompers, JJ 2012, In vivo magnetic resonance spectroscopy of lipid handling in steatotic rat liver. in Experimental Biology 2012 : San Diego, April 21-25, 2012. FASEB Journal : The Journal of the Federation of American Societies for Experimental Biology, vol. 26, pp. 242.7-, conference; Experimental Biology MeetingLocation:San Diego, CADate:APR 21-25, 2012, 1/01/12.

In vivo magnetic resonance spectroscopy of lipid handling in steatotic rat liver. / Janssens, S.M.M.; Jonkers, R.A.M.; Riel, van, N.A.W.; Nicolay, K.; Prompers, J.J.

Experimental Biology 2012 : San Diego, April 21-25, 2012. 2012. p. 242.7- (FASEB Journal : The Journal of the Federation of American Societies for Experimental Biology; Vol. 26).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - In vivo magnetic resonance spectroscopy of lipid handling in steatotic rat liver

AU - Janssens,S.M.M.

AU - Jonkers,R.A.M.

AU - Riel, van,N.A.W.

AU - Nicolay,K.

AU - Prompers,J.J.

PY - 2012

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N2 - Objective: Examine lipid handling in liver of rats fed with different high-fat diets using 1H-[13C] magnetic resonance spectroscopy (MRS) together with oral administration of 13C labeled lipids. Methods: 6 male Wistar rats (11 weeks old; 348 ± 8g) were divided into three diet groups: low-fat (10% fat, CON), high-fat lard (45% fat, HFL), and high-fat palm oil (45%, HFP). After 10 weeks of diet, MRS experiments were performed at baseline, and 4 and 24 h after oral administration of 1.5 g [U-13C] Algal lipid mixture per kg body weight. Results: At 4 h after administration of the 13C labeled lipids, 13C enrichment of intracellular liver lipids was similarly increased in all three groups compared to baseline (CON: 0.031 ± 0.017 %; HFL: 0.045 ± 0.022 %; HFP: 0.033 ± 0.013 %), demonstrating that lipid uptake was not affected by the diet regimen. At 24 h, on the other hand, 13C enrichment of liver lipids decreased in CON, whereas in both high-fat diet groups the 13C enrichment did not change compared to 4 h, indicating a lower turnover of the stored liver lipids. Conclusion: High-fat diet feeding did not alter liver lipid uptake in rats, but resulted in a decreased turnover of the lipids stored in the liver. This research was funded by the Netherlands Consortium for Systems Biology (NCSB) which is part of the Netherlands Genomics Initiative/Netherlands Organisation for Scientific Research.

AB - Objective: Examine lipid handling in liver of rats fed with different high-fat diets using 1H-[13C] magnetic resonance spectroscopy (MRS) together with oral administration of 13C labeled lipids. Methods: 6 male Wistar rats (11 weeks old; 348 ± 8g) were divided into three diet groups: low-fat (10% fat, CON), high-fat lard (45% fat, HFL), and high-fat palm oil (45%, HFP). After 10 weeks of diet, MRS experiments were performed at baseline, and 4 and 24 h after oral administration of 1.5 g [U-13C] Algal lipid mixture per kg body weight. Results: At 4 h after administration of the 13C labeled lipids, 13C enrichment of intracellular liver lipids was similarly increased in all three groups compared to baseline (CON: 0.031 ± 0.017 %; HFL: 0.045 ± 0.022 %; HFP: 0.033 ± 0.013 %), demonstrating that lipid uptake was not affected by the diet regimen. At 24 h, on the other hand, 13C enrichment of liver lipids decreased in CON, whereas in both high-fat diet groups the 13C enrichment did not change compared to 4 h, indicating a lower turnover of the stored liver lipids. Conclusion: High-fat diet feeding did not alter liver lipid uptake in rats, but resulted in a decreased turnover of the lipids stored in the liver. This research was funded by the Netherlands Consortium for Systems Biology (NCSB) which is part of the Netherlands Genomics Initiative/Netherlands Organisation for Scientific Research.

M3 - Conference contribution

T3 - FASEB Journal : The Journal of the Federation of American Societies for Experimental Biology

SP - 242.7-

BT - Experimental Biology 2012 : San Diego, April 21-25, 2012

ER -

Janssens SMM, Jonkers RAM, Riel, van NAW, Nicolay K, Prompers JJ. In vivo magnetic resonance spectroscopy of lipid handling in steatotic rat liver. In Experimental Biology 2012 : San Diego, April 21-25, 2012. 2012. p. 242.7-. (FASEB Journal : The Journal of the Federation of American Societies for Experimental Biology).